Umfang:
19
ISSN:
1934-6069
Inhalt:
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome. We leveraged the compact nature of this library to systematically screen SARS-CoV-2 at two physiologically relevant temperatures along with three related coronaviruses (human coronavirus 229E [HCoV-229E], HCoV-NL63, and HCoV-OC43), allowing us to probe this interactome at a much higher resolution than genome-scale studies. This approach yielded several insights, including potential virus-specific differences in Rab GTPase requirements and glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis. This coronavirus essentiality catalog could inform ongoing drug development efforts aimed at intercepting and treating coronavirus disease 2019 (COVID-19) and help prepare for future coronavirus outbreaks.
Anmerkung:
Available online 16 December 2020
,
Gesehen am 10.05.2021
In:
Cell host and microbe, Amsterdam [u.a.] : Elsevier, 2007, 29(2021), 2, Seite 267-280.e5, 1934-6069
In:
volume:29
In:
year:2021
In:
number:2
In:
pages:267-280.e5
In:
extent:19
Sprache:
Englisch
DOI:
10.1016/j.chom.2020.12.009
URL:
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