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  • 1
  • 2
    UID:
    gbv_427132495
    Format: 55 S.: graph. Darst.; 8"
    Note: München, Univ., 07-Fachber. Med., Diss. 1980
    Language: Undetermined
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    UID:
    b3kat_BV024601121
    Format: 167 S. , Ill., graph. Darst.
    Note: Göttingen, Univ., Diss., 1988
    Language: Undetermined
    Keywords: Hochschulschrift
    Library Location Call Number Volume/Issue/Year Availability
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  • 4
    UID:
    gbv_275080048
    Format: 167 S , Ill., graph. Darst
    Note: Göttingen, Univ., Diss., 1988
    Language: Undetermined
    Keywords: Hochschulschrift
    Library Location Call Number Volume/Issue/Year Availability
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  • 5
    UID:
    almafu_BV026079921
    Format: 167 S. : Ill., graph. Darst.
    Note: Göttingen, Univ., Diss., 1988
    Keywords: Ratte ; MHC ; Gen ; Hochschulschrift
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  • 6
    UID:
    almahu_BV001257821
    Format: 55 S. : graph. Darst.
    Note: München, Univ., Diss.
    Language: German
    Keywords: Hochschulschrift
    Library Location Call Number Volume/Issue/Year Availability
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  • 7
    UID:
    almahu_BV002197393
    Format: 167 S. : Ill., graph. Darst.
    Note: Göttingen, Univ., Diss.
    Language: German
    Keywords: Ratte ; MHC ; Gen ; Hochschulschrift
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  • 8
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    edoccha_9959000182602883
    Format: 1 online resource (XVI, 516 p.)
    Edition: 2nd ed. 2009.
    ISBN: 1-59745-471-0
    Series Statement: Methods in Molecular Biology, 530
    Content: With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
    Note: Bibliographic Level Mode of Issuance: Monograph , Gene Modification in ES Cells -- Overview on Mouse Mutagenesis -- Construction of Gene-Targeting Vectors by Recombineering -- Gene-Trap Vectors and Mutagenesis -- Chromosome Engineering in ES Cells -- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides -- Generation of shRNA Transgenic Mice -- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate -- Stem Cell Manipulation -- Gene Targeting in Mouse Embryonic Stem Cells -- Manipulating Mouse Embryonic Stem Cells -- ES Cell Line Establishment -- Generation of Double-Knockout Embryonic Stem Cells -- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro -- Cloning of ES Cells and Mice by Nuclear Transfer -- Genetically Engineered Mice -- Isolation, Microinjection and Transfer of Mouse Blastocysts -- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos -- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos -- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes -- Inducible Cre Mice -- Creation and Use of a Cre Recombinase Transgenic Database -- Transposon Mutagenesis in Mice -- Lentiviral Transgenesis -- Sperm Cryopreservation and In Vitro Fertilization -- Phenotype Analysis -- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes -- Pathologic Phenotyping of Mutant Mice -- Systemic First-Line Phenotyping. , English
    Additional Edition: ISBN 1-934115-26-6
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 9
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    edocfu_9959000182602883
    Format: 1 online resource (XVI, 516 p.)
    Edition: 2nd ed. 2009.
    ISBN: 1-59745-471-0
    Series Statement: Methods in Molecular Biology, 530
    Content: With the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
    Note: Bibliographic Level Mode of Issuance: Monograph , Gene Modification in ES Cells -- Overview on Mouse Mutagenesis -- Construction of Gene-Targeting Vectors by Recombineering -- Gene-Trap Vectors and Mutagenesis -- Chromosome Engineering in ES Cells -- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides -- Generation of shRNA Transgenic Mice -- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate -- Stem Cell Manipulation -- Gene Targeting in Mouse Embryonic Stem Cells -- Manipulating Mouse Embryonic Stem Cells -- ES Cell Line Establishment -- Generation of Double-Knockout Embryonic Stem Cells -- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro -- Cloning of ES Cells and Mice by Nuclear Transfer -- Genetically Engineered Mice -- Isolation, Microinjection and Transfer of Mouse Blastocysts -- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos -- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos -- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes -- Inducible Cre Mice -- Creation and Use of a Cre Recombinase Transgenic Database -- Transposon Mutagenesis in Mice -- Lentiviral Transgenesis -- Sperm Cryopreservation and In Vitro Fertilization -- Phenotype Analysis -- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes -- Pathologic Phenotyping of Mutant Mice -- Systemic First-Line Phenotyping. , English
    Additional Edition: ISBN 1-934115-26-6
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 10
    Online Resource
    Online Resource
    New York, NY :Springer New York :
    UID:
    edocfu_9959000006602883
    Format: 1 online resource (XII, 274 p. 65 illus., 50 illus. in color.)
    Edition: 1st ed. 2016.
    ISBN: 1-4939-2932-1
    Series Statement: Methods in Molecular Biology, 1338
    Content: This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.
    Note: Bibliographic Level Mode of Issuance: Monograph , Xanthomonas and the TAL effectors:  Nature's Molecular Biologist -- TAL Effector DNA-binding Principles and Specificity -- The Development of TALE Nucleases for Biotechnology -- Online Tools for TALEN Design -- Assembly of Customized TAL Effectors through Advanced ULtiMATE System -- Engineering Customized TALENs Using the Platinum Gate TALEN Kit -- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors -- A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV) -- TALEN Induced Translocations in Human Cells -- Mutagenesis in Newts; Protocol for Iberian Ribbed Newts -- Targeted Mutagenesis in Bombyx mori Using TALENs -- GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs -- Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti -- Methods for TALEN-mediated Genomic Manipulations in Drosophila -- Targeted Mutagenesis in Zebrafish by TALENs -- Mutagenesis in Xenopus and Zebrafish using TALENs -- Genome Editing in Mice using TALE Nucleases -- Genome Editing in Rats using TALE Nucleases -- Designer Nuclease-mediated Generation of Knock out THP1 Cells. , English
    Additional Edition: ISBN 1-4939-2931-3
    Language: English
    Keywords: Laboratory Manuals ; Laboratory manuals.
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