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Berlin Brandenburg

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  • 2000  (15)
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  • 2000  (15)
  • 1
    In: The Journal of Cell Biology, Feb 7, 2000, Vol.148(3), p.567
    Description: The anterior-posterior axis of the mouse embryo is defined before formation of the primitive streak, and axis specification and subsequent anterior development involves signaling from both embryonic ectoderm and visceral endoderm. The Wnt signaling pathway is essential for various developmental processes, but a role in anterior-posterior axis formation in the mouse has not been previously established. [Beta]-Catenin is a central player in the Wnt pathway and in cadherin-mediated cell adhesion. We generated [Beta]-catenin-deficient mouse embryos and observed a defect in anterior-posterior axis formation at embryonic day 5.5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression. Subsequently, no mesoderm and head structures are generated. Intercellular adhesion is maintained since plakoglobin substitutes for [Beta]-catenin. Our data demonstrate that [Beta]-catenin function is essential in anterior-posterior axis formation in the mouse, and experiments with chimeric embryos show that this function is required in the embryonic ectoderm. Key words: anterior visceral endoderm * Wnt/wingless pathway * cell adhesion * plakoglobin * armadillo
    Keywords: Cell Adhesion -- Research ; Armadillos -- Research
    ISSN: 0021-9525
    E-ISSN: 15408140
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  • 2
    In: Biology of Reproduction, 2000, Vol.63(5), p.1555-1561
    Description: Abstract We developed a novel promoter-based selection strategy that could be used to produce cell lines representing sequential stages of spermatogenesis. The method is based on immortalization and subsequent targeted selection by using differentiation-specific promoter regions. As an example for this approach, a new murine germ cell line (GC-4spc) was established using a vector construct that contains the SV40 large T antigen and the neomycin phosphotransferase II gene under the control of the SV40 early promoter and a spermatocyte-specific promoter for human phosphoglycerate kinase 2, respectively. The GC-4spc was characterized as a cell line at the stage between preleptotene and early pachytene spermatocytes. Transcription of three germ cell-specific expressed genes, Pgk2, proacrosin, and the A-myb proto-oncogene, were detected in the GC-4spc cell line using reverse transcription-polymerase chain reaction. Furthermore, TSPY (human testis-specific protein, Y-encoded) and PGK2 (human phosphoglycerate kinase 2) promoter regions showed different transcriptional activities in the GC-4spc cell line compared with the spermatogonia-derived cell line GC-1spg. Thus, our strategy could be used for immortalization of cells at specific stages of differentiation, allowing production of a series of cultured cell lines representing sequential stages of differentiation in given cell lineages.
    Keywords: Spermatogenesis ; Testes
    ISSN: 0006-3363
    E-ISSN: 15297268
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  • 3
    In: Angewandte Chemie International Edition, 4/17/2000, Vol.39(8), pp.1453-1455
    ISSN: 14337851
    E-ISSN: 15213773
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  • 4
    Language: English
    In: Angewandte Chemie International Edition, 17 April 2000, Vol.39(8), pp.1453-1455
    Description: : 4‐(‐methylhydrazino)‐7‐nitro‐2,1,3‐benzooxadiazole (MNBDH; ) is introduced as a prototype for a new class of fluorogenic peroxide substrates. The advantages of lie in its lower detection and quantification limits, a favorable red‐shifted fluorescence signal, and a greater signal stability after the successful reaction. The new substrate was validated by the determination of ‐‐glucose in beverages.
    Keywords: Analytical Methods ; Enzyme Catalysis ; Fluoroscence Spectroscopy
    ISSN: 1433-7851
    E-ISSN: 1521-3773
    Source: John Wiley & Sons, Inc.
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  • 5
    In: The Journal of Cell Biology, 2000, Vol.148(3), p.567-578
    Description: The anterior-posterior axis of the mouse embryo is defined before formation of the primitive streak, and axis specification and subsequent anterior development involves signaling from both embryonic ectoderm and visceral endoderm. Τhe Wnt signaling pathway is essential for various developmental processes, but a role in anterior-posterior axis formation in the mouse has not been previously established. β-Catenin is a central player in the Wnt pathway and in cadherin-mediated cell adhesion. We generated β-catenin –deficient mouse embryos and observed a defect in anterior-posterior axis formation at embryonic day 5.5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression. Subsequently, no mesoderm and head structures are generated. Intercellular adhesion is maintained since plakoglobin substitutes for β-catenin. Our data demonstrate that β-catenin function is essential in anterior-posterior axis formation in the mouse, and experiments with chimeric embryos show that this function is required in the embryonic ectoderm.
    Keywords: Original Article ; Anterior Visceral Endoderm ; Wnt/Wingless Pathway ; Cell Adhesion ; Plakoglobin ; Armadillo
    ISSN: 0021-9525
    E-ISSN: 1540-8140
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  • 6
    Language: English
    In: Ophelia, 01 March 2000, Vol.52(1), pp.57-64
    Description: Colour morphs of Anemonia aff. silicata, var. rustica, var. vulgaris, var. alinda, var. alabastrina and var. smaragdina (ecotype II) were found in close neighbourhood in a sheltered bay of the Mediterranean island Giglio in the Thyrrhenian Sea. Some of the morphs are known to form colonies of genetically identical individuals by asexual propagation. Thus, the complex population in the studied area was analysed by DNA fingerprinting to reveal or exclude a genetic basis for the phenotypic differences. To this end a modified technique for DNA preparation has been established which ensures survival of the animals and may be well suited for other studies in Actinaria. The morphs var. rustica and var. vulgaris were represented by one clone each consisting of an enormous number of animals. The specimens of the morphs var. alinda and var. alabastrina were also members of these clones as evidenced by their genotypes. They are characterised by a low content of pigments of zooxanthellae and, thus, do not represent real colour morphs. The reddish and green tones of tentacles of var. vulgaris and var. smaragdina seem to be independent from the content of zooxanthellae, which is in contrast to earlier statements. Furthermore, all individuals of var. smaragdina (ecotype II) were genetically different, which points to a lack of asexual reproduction in this morph.
    Keywords: Oceanography ; Ecology
    ISSN: 0078-5326
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  • 7
    Language: German
    In: Angewandte Chemie, 17 April 2000, Vol.112(8), pp.1510-1512
    Description: : Mit 4‐(‐Methylhydrazino)‐7‐nitro‐2,1,3‐benzooxadiazol (MNBDH) wird der Prototyp einer neuen Klasse fluorogener Peroxidasesubstrate vorgestellt. Vorteile der Verwendung von liegen in den gegenüber anderen Substraten deutlich niedrigeren Nachweis‐ und Bestimmungsgrenzen, der günstigen Rotverschiebung der Anregungs‐ und Emissionsmaxima und der größeren Signalstabilität nach erfolgter Reaktion. Das neue Substrat wurde anhand der Bestimmung von α‐‐Glucose in Getränken validiert.
    Keywords: Analytische Methoden ; Enzymkatalyse ; Fluoreszenzspektroskopie
    ISSN: 0044-8249
    E-ISSN: 1521-3757
    Source: John Wiley & Sons, Inc.
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  • 8
    Language: English
    In: High Temperatures-High Pressures, 2000, Vol.32(1), pp.73-81
    ISSN: 0018-1544
    E-ISSN: 1472-3441
    Source: CrossRef
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  • 9
    Language: German
    In: Angewandte Chemie, 04/17/2000, Vol.112(8), pp.1510-1512
    ISSN: 0044-8249
    E-ISSN: 1521-3757
    Source: Wiley (via CrossRef)
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  • 10
    Language: English
    In: Immunopharmacology, 8/2000, Vol.49(1-2), p.94
    ISSN: 01623109
    Source: Elsevier (via CrossRef)
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