Format:
1 Online-Ressource
,
illustrations
ISBN:
9781316403938
,
9781108696616
Series Statement:
Advances in microscopy and microanalysis
Content:
Cover; Half-title page; Series page; Title page; Copyright page; Dedication; Contents; List of Contributors; Preface; Notation; Part I Adaptive Optical Microscopy for Biological Imaging; 1 Adaptive Optical Microscopy Using Image-Based Wavefront Sensing; 1.1 Introduction; 1.2 Simple Sensorless Adaptive Optics System; 1.3 Image-Based Adaptive Optics Systems; 1.4 Conclusion; 1.5 References; 2 Adaptive Optical Microscopy Using Guide Star-Based Direct Wavefront Sensing; 2.1 Introduction; 2.2 Background; 2.3 Techniques; 2.4 Conclusions and Future Directions; 2.5 References
Content:
Part II Deep Tissue Microscopy3 Deep Tissue Fluorescence Microscopy; 3.1 Introduction; 3.2 Background; 3.3 Results; 3.4 Discussion; 3.5 References; 4 Zonal Adaptive Optical Microscopy for Deep Tissue Imaging; 4.1 Introduction; 4.2 Adaptive Optics in Microscopy; 4.3 Zonal Adaptive Optical Two-Photon Microscopy with Direct Wavefront Sensing; 4.4 Pupil-Segmentation AO Microscopy with Single-Segment Illumination; 4.5 Pupil Segmentation AO with Full-Pupil Illumination; 4.6 Discussion; 4.7 References; Part III Focusing Light through Turbid Media Using the Scattering Matrix
Content:
5 Transmission Matrix Approach to Light Control in Complex Media5.1 Introduction; 5.2 Techniques; 5.3 Results; 5.4 Recent Developments; 5.5 Future Directions; 5.6 References; 6 Coupling Optical Wavefront Shaping and Photoacoustics; 6.1 Introduction; 6.2 Principles of Photacoustics; 6.3 Photoacoustic-Guided Optical Wavefront Shaping; 6.4 Wavefront Shaping for Minimally Invasive Photoacoustic Microendoscopy; 6.5 Conclusions and Future Directions; 6.6 References; 7 Imaging and Controlling Light Propagation Deep within Scattering Media Using a Time-Resolved Reflection Matrix; 7.1 Introduction
Content:
7.2 Time Domain Measurements of the Time-Resolved Reflection Matrix Measurements7.3 Spectral Domain Measurements of the Time-Resolved Reflection Matrix; 7.4 Summary and Future Outlook; 7.5 References; Part IV Focusing Light through Turbid Media Using Feedback Optimization; 8 Feedback-Based Wavefront Shaping; 8.1 Introduction; 8.2 Matrix Formalism; 8.3 Wavefront Shaping; 8.4 Algorithms; 8.5 Extended Targets; 8.6 Feedback; 8.7 Correlations and Dynamics; 8.8 Related Topics; 8.9 Applications and Outlook; 8.10 References
Content:
9 Focusing Light through Scattering Media Using a Microelectromechanical Systems Spatial Light Modulator9.1 Introduction; 9.2 Background on MEMS SLM; 9.3 MEMS SLM for Dynamic Scattering Media; 9.4 MEMS SLM for Scattered Light Control in Multiphoton Microscopy; 9.5 Future Direction: Field of View Enhancement by Conjugate Adaptive Optics; 9.6 References; 10 Computer-Generated Holographic Techniques to Control Light Propagating through Scattering Media Using a Digital-Mirror-Device Spatial Light Modulator; 10.1 Introduction; 10.2 Binary Holographic Techniques for Phase Control
Content:
"The most common approach to adaptive optics (AO), as originally employed in astronomical telescopes, has been to use a wavefront sensor to measure directly aberrations. In situations where such sensing provides reliable measurement, this is clearly the ideal method (see Chapter 2), but this approach has limitations, and particularly so in the context of microscopy. In order to understand this, one should consider the constraints the use of a wavefront sensor places on the nature of the optical conguration. A wavefront is only well de ned in particular situations, for example when light is emitted by a small or distant, point-like object, such as a star for a telescope or a minuscule bead in a microscope. In these situations, a wavefront sensor provides a clear and reliable measurement and this phenomenon has been used to great effect, as explained in Chapter 2. However, not all sources of light have these necessary properties. For example, a large luminous object comprises an arrangement of individual emitters, each of which produces its associated wavefront. In this case, a wavefront sensor would respond to all of the light impinging upon it, thus giving potentially ambiguous measurements. In an extreme case, such as where light is emitted throughout the volume of the specimen, the sensor would be swamped with light and thus be un-able to provide sensible aberration measurement. For this reason, in microscopy, direct wavefront sensing has been e ective where point-like sources have been employed, either through the introduction of uorescent beads [1, 2], or using localised uorescent markers [3] and non-linear excited guide stars [4, 5, 6]"--Provided by publisher
Note:
Includes bibliographical references and index
Additional Edition:
ISBN 9781107124127
Additional Edition:
Erscheint auch als Druck-Ausgabe ISBN 9781107124127
Language:
English
Keywords:
Electronic books
DOI:
10.1017/9781316403938
URL:
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