Format:
Online-Ressource
ISSN:
1439-7633
Content:
Abstract: During placental formation, cytotrophoblasts (CTBs) fuse into multinucleate, microvilli‐coated syncytiotrophoblasts (STBs), which contact maternal blood, mediating nutrient, metabolite, and gas exchange between mother and fetus, and providing a barrier against fetal infection. Trophoblasts remodel the surrounding extracellular matrix through the secretion of matrix metalloproteinases (MMPs). Maternal obesity and diabetes mellitus can negatively impact fetal development and may impair trophoblast function. We sought to model the impact of metabolic stress on STB function by examining MMP and hormone secretion. The BeWo CTB cell line was syncytialized to STB‐like cells with forskolin. Cell morphology was examined by electron microscopy and immunofluorescence; phenotype was further assessed by ELISA and RT‐qPCR. STBs were exposed to a metabolic stress cocktail (MetaC: 30 mM glucose, 10 nM insulin, and 0.1 mM palmitic acid). BeWo syncytialization was demonstrated by increased secretion of HCGβ and progesterone, elevated syncytin gene expression (ERVW‐1 and ERVFRD‐1), loss of tight junctions, and increased surface microvilli. MetaC strongly suppressed syncytin gene expression (ERVW‐1 and ERVFRD‐1), suppressed HCGβ and progesterone secretion, and altered both MMP‐9 and MMP‐2 production. Metabolic stress modeling diabetes and obesity altered BeWo STB hormone and MMP production in vitro.
In:
day:24
In:
month:10
In:
year:2023
In:
extent:9
In:
ChemBioChem, Weinheim : Wiley-VCH, [2000]-, (24.10.2023) (gesamt 9), 1439-7633
Language:
English
DOI:
10.1002/cbic.202300410
URN:
urn:nbn:de:101:1-2023102515132565496081
URL:
https://doi.org/10.1002/cbic.202300410
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023102515132565496081
URL:
https://d-nb.info/1307252575/34
URL:
https://doi.org/10.1002/cbic.202300410
Bookmarklink