ISSN:
1757-4749
Content:
Introduction: Quinolone prophylaxis is recommended for patients with advanced cirrhosis at high risk of spontaneous bacterial peritonitis (SBP) or with prior SBP. Yet, the impact of long-term antibiotic prophylaxis on the microbiome of these patients is poorly characterized. Methods: Patients with liver cirrhosis receiving long-term quinolone prophylaxis to prevent SBP were prospectively included and sputum and stool samples were obtained at baseline, 1, 4 and 12 weeks thereafter. Both bacterial DNA and RNA were assessed with 16S rRNA sequencing. Relative abundance, alpha and beta diversity were calculated and correlated with clinical outcome. Results: Overall, 35 stool and 19 sputum samples were obtained from 11 patients. Two patients died (day 9 and 12) all others were followed for 180 days. Reduction of Shannon diversity and bacterial richness was insignificant after initiation of quinolone prophylaxis (p 〉 0.05). Gut microbiota were significantly different between patients (p 〈 0.001) but non-significantly altered between the different time points before and after initiation of antibiotic prophylaxis (p 〉 0.05). A high relative abundance of Enterobacteriaceae 〉 20% during quinolone prophylaxis was found in three patients. Specific clinical scenarios (development of secondary infections during antibiotic prophylaxis or the detection of multidrug-resistant Enterobacteriaceae) characterized these patients. Sputum microbiota were not significantly altered in individuals during prophylaxis. Conclusion: The present exploratory study with small sample size showed that inter-individual differences in diversity of gut microbiota were high at baseline, yet quinolone prophylaxis had only a moderate impact. High relative abundances of Enterobacteriaceae during follow-up might indicate failure of or non-adherence to quinolone prophylaxis. However, our results may not be clinically significant given the limitations of the study and therefore future studies are needed to further investigate this phenomenon.
In:
Gut pathogens, London : BioMed Central, 2009-, Band 12 (2020), Seite 1-10, Artikel-ID: 51, 1757-4749
In:
volume:12
In:
year:2020
In:
pages:1-10
In:
extent:10
In:
elocationid:51
Language:
English
DOI:
10.1186/s13099-020-00389-y
URN:
urn:nbn:de:hebis:30:3-628152
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