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1,3‐ Alternate calix (4) arene‐bonded silica stationary phases. Effect of calixarene skeleton substituents on the retention mechanism and column selectivity (2009)

Śliwka‐Kaszyńska, Magdalena [Verfasser] ; Jaszcołt, Katarzyna [Verfasser] ; Anusiewicz, Iwona [Verfasser]

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UID:

(DE-101)1286598419

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: Four novel 1,3‐alternate calix[4]arene‐bonded silica gel stationary phases possessing different aromatic and aliphatic substituents at the upper rim (CalixNph, CalixBph, CalixHex, and CalixDdc) were prepared and structurally characterized. The comparison and selectivity of these phases were done by using alkylbenzenes, fatty acid p‐bromophenacyl esters, aromatic positional isomers, and polynuclear aromatic hydrocarbons as analytes. Quantum chemistry calculations have also been performed (using an ab initio method) to support the experimental findings. The effect of the type and content of organic modifier on the retention and selectivity of the alkylbenzenes was studied. The retention mechanism is also discussed. The results indicate that the stationary phases behave like RP packings. However, inclusion complex formation and hydrophobic and π–π interactions seem to be involved in the separation process.

In: volume:32

In: number:18

In: year:2009

In: pages:3107-3115

In: extent:9

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 32, Heft 18 (2009), 3107-3115 (gesamt 9), 1615-9314

Language: English

DOI: 10.1002/jssc.200900330

URN: urn:nbn:de:101:1-2023041909341183290612

URL: https://doi.org/10.1002/jssc.200900330

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041909341183290612

URL: https://d-nb.info/1286598419/34

URL: https://doi.org/10.1002/jssc.200900330

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1 H HR/MAS NMR in the suspended state: Molecular recognition processes in liquid chromatography between steroids and a silica hydride‐based cholesterol phase (2009)

Friebolin, Volker [Verfasser] ; Bayer, Marc P. [Verfasser] ; Matyska, Maria T. [Verfasser] ; [et al.]

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UID:

(DE-101)1287024483

Format: Online-Ressource

ISSN: 1615-9314

In: volume:32

In: number:10

In: year:2009

In: pages:1722-1728

In: extent:7

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 32, Heft 10 (2009), 1722-1728 (gesamt 7), 1615-9314

Language: English

DOI: 10.1002/jssc.200800697

URN: urn:nbn:de:101:1-2023042605555970471250

URL: https://doi.org/10.1002/jssc.200800697

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023042605555970471250

URL: https://d-nb.info/1287024483/34

URL: https://doi.org/10.1002/jssc.200800697

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1 mm ID poly (styrene‐ co ‐divinylbenzene) monolithic columns for high‐peak capacity one‐ and two‐dimensional liquid chromatographic separations of intact proteins (2009)

Eeltink, Sebastiaan [Verfasser] ; Dolman, Sebastiaan [Verfasser] ; Detobel, Frederik [Verfasser] ; [et al.]

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UID:

(DE-101)128660186X

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: The LC performance of a 1×50 mm polymer monolithic column format was demonstrated with high‐peak capacity one‐ (1D) and offline two dimensional (2D) LC separations of intact proteins. After optimizing the RP 1D‐LC conditions, including column temperature, flow rate and gradient time, a peak capacity of 475 was achieved within a 2‐h analysis. The suitability of the monolithic column was also demonstrated for fast 1 min protein separations yielding 1 s peak widths determined at half peak height. In addition, an offline 2D‐LC method was developed using the micro‐fraction collection capabilities of the autosampler allowing automatic fractionation of intact proteins after the weak‐ion‐exchange (WAX) separation, and re‐injection of the fractions onto the second‐dimension RP monolithic column. The best peak capacity‐to‐analysis time ratio was obtained when applying 10 min second‐dimension RP gradients. At optimized conditions, the WAX/×/RPLC separation of intact Escherichia coli proteins was performed within 6 h yielding a maximum theoretical peak capacity of 4880.

In: volume:32

In: number:15‐16

In: year:2009

In: pages:2504-2509

In: extent:6

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 32, Heft 15‐16 (2009), 2504-2509 (gesamt 6), 1615-9314

Language: English

DOI: 10.1002/jssc.200900068

URN: urn:nbn:de:101:1-2023041910012188436453

URL: https://doi.org/10.1002/jssc.200900068

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041910012188436453

URL: https://d-nb.info/128660186X/34

URL: https://doi.org/10.1002/jssc.200900068

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15th International Symposium on Microscale Separations and Analysis, HPCE 2002 Stockholm, Sweden, April 14–18, 2002 (2002)

Kašička, Václav [Verfasser]

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UID:

(DE-101)1307176739

Format: Online-Ressource

ISSN: 1615-9314

In: volume:25

In: number:8

In: year:2002

In: pages:547-548

In: extent:2

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 25, Heft 8 (2002), 547-548 (gesamt 2), 1615-9314

Language: English

DOI: 10.1002/1615-9314(20020601)25:8〈547::AID-JSSC547〉3.0.CO;2-K

URN: urn:nbn:de:101:1-2023102504304433843546

URL: https://doi.org/10.1002/1615-9314(20020601)25:8〈547::AID-JSSC547〉3.0.CO;2-K

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023102504304433843546

URL: https://d-nb.info/1307176739/34

URL: https://doi.org/10.1002/1615-9314(20020601)25:8〈547::AID-JSSC547〉3.0.CO;2-K

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2,6‐Dimethyl‐4‐quinolinecarboxylic acid N ‐hydroxysuccinimide ester: A fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines (2006)

Wang, Hong [Verfasser] ; Liu, Xin [Verfasser] ; Zhang, Niu [Verfasser] ; [et al.]

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UID:

(DE-101)1300639245

Format: Online-Ressource

ISSN: 1615-9314

In: volume:29

In: number:5

In: year:2006

In: pages:635-640

In: extent:6

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 29, Heft 5 (2006), 635-640 (gesamt 6), 1615-9314

Language: English

DOI: 10.1002/jssc.200500508

URN: urn:nbn:de:101:1-2023090104482018944546

URL: https://doi.org/10.1002/jssc.200500508

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023090104482018944546

URL: https://d-nb.info/1300639245/34

URL: https://doi.org/10.1002/jssc.200500508

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2‐D chromatography applied to the study of block copolymers synthesized by nitroxide‐mediated controlled free‐radical seeded emulsion polymerization (2010)

Moyses, Stephan [Verfasser] ; Ness, Jason [Verfasser] ; Papakonstantopoulos, George [Verfasser]

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UID:

(DE-101)1286252156

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: 2‐D chromatography with gradient polymer elution chromatography in the first dimension was used to characterize a series of block copolymers prepared by nitroxide‐mediated controlled free‐radical seeded emulsion polymerization. The data provide a detailed picture of the different populations generated during the successive stages of the reaction. In particular, deviations from ideal conditions, such as an exothermic temperature spike, are obvious from the 2‐D chromatogram, and its interpretation provides unique insight into the reaction mechanisms. Finally, atomic force microscopy images obtained on films cast from copolymer solutions evidence the relationship between polymer microstructure and film morphology.

In: volume:33

In: number:22

In: year:2010

In: pages:3511-3518

In: extent:8

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 33, Heft 22 (2010), 3511-3518 (gesamt 8), 1615-9314

Language: English

DOI: 10.1002/jssc.201000524

URN: urn:nbn:de:101:1-2023041411395580079064

URL: https://doi.org/10.1002/jssc.201000524

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041411395580079064

URL: https://d-nb.info/1286252156/34

URL: https://doi.org/10.1002/jssc.201000524

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2‐D chromatography with optimized size exclusion chromatography resolution and multi‐angle light scattering coupling (2010)

Moyses, Stephan [Verfasser]

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UID:

(DE-101)1286490545

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: 2‐D chromatography has become an important tool for the polymer chemist and engineer interested in understanding reaction mechanisms in multiple step polymerization reactions and for establishing the relationships between structure and property. As with 1‐D chromatography, it is desirable to obtain the best possible resolution in order to distinguish subtle differences in composition and molecular weight. This requirement is in conflict with the need to keep the duration of the experiment short enough for practical purposes. In this paper, we use a model system to investigate the optimum conditions for improved 2‐D resolution with a focus on the molecular weight dimension. This dimension is usually the less resolved in 2‐D systems due to synchronization requirements between the two separation processes and the subsequent need to use a rapid size exclusion chromatography column. The method described here provides resolution in the SEC dimension equivalent to that of a standard 1‐D SEC method, while maintaining a relatively short analysis time. Finally, a multi‐angle light scattering detector was used to determine the radius of gyration of the eluting polymer.

In: volume:33

In: number:10

In: year:2010

In: pages:1480-1486

In: extent:7

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 33, Heft 10 (2010), 1480-1486 (gesamt 7), 1615-9314

Language: English

DOI: 10.1002/jssc.201000009

URN: urn:nbn:de:101:1-2023041806213793290332

URL: https://doi.org/10.1002/jssc.201000009

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041806213793290332

URL: https://d-nb.info/1286490545/34

URL: https://doi.org/10.1002/jssc.201000009

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2‐D hydrophilic interaction liquid chromatography‐RP separation in urinary proteomics – Minimizing variability through improved downstream workflow compatibility (2009)

Loftheim, Håvard [Verfasser] ; Nguyen, Thien Duc [Verfasser] ; Malerød, Helle [Verfasser] ; [et al.]

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UID:

(DE-101)1286620333

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: Optimization of every step in a bottom‐up urinary proteomics approach was studied with respect to maximize the protein recovery and making the downstream steps in the workflow fully compatible without compromising on the amount of information obtained. Sample enrichment and desalting using centrifugal filtration (5 kDa cut‐off) yielded protein recoveries up to 97% when 8 M urea was used. Although yielding lower recoveries (88%), addition of Tris‐HCl/NaCl was considered a better choice due to good down‐stream compatibility. The consecutive depletion of HSA, using an immunoaffinity column was successfully adapted for use in urine. Separation of the trypsin generated peptides in an off‐line 2‐D chromatographic system consisting of a hydrophilic interaction liquid chromatography column, followed by a RP chromatography column showed a high peak capacity and good repeatability in addition to a high degree of orthogonality. All operations were modified in order to keep sample handling between every step to a minimum, reducing the variability of each process. In order to test the suitability of the full method in an extensive proteomic experiment, a urine sample from a kidney‐transplanted patient was analyzed (n=6). The total variability of the method was identified with RSD values ranging from 11 to 30%. Eventually, we identified a total of 1668 peptides and 438 proteins from a single urine sample despite the use of low‐resolution MS/MS equipment. The optimized and “streamlined” complex method has shown potential for use in future urinary proteomic studies.

In: volume:33

In: number:6‐7

In: year:2010

In: pages:864-872

In: extent:9

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 33, Heft 6‐7 (2010), 864-872 (gesamt 9), 1615-9314

Language: English

DOI: 10.1002/jssc.200900554

URN: urn:nbn:de:101:1-2023041911582865624449

URL: https://doi.org/10.1002/jssc.200900554

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041911582865624449

URL: https://d-nb.info/1286620333/34

URL: https://doi.org/10.1002/jssc.200900554

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2‐D RP/RPLC method to separate components in Fructus schisandrae chinensis (2010)

Jin, Gaowa [Verfasser] ; Dai, Yuntao [Verfasser] ; Feng, Jiatao [Verfasser] ; [et al.]

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UID:

(DE-101)1286587352

Format: Online-Ressource

ISSN: 1615-9314

Content: Abstract: An off‐line 2‐D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click β‐CD stationary phase was proposed for constructing 2‐D RP/RPLC system combined with C18 column. β‐CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click β‐CD column used in first dimension were resolved into many additional components, indicating that the 2‐D LC system based on Click β‐CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2‐D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.

In: volume:33

In: number:4‐5

In: year:2010

In: pages:564-569

In: extent:6

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 33, Heft 4‐5 (2010), 564-569 (gesamt 6), 1615-9314

Language: English

DOI: 10.1002/jssc.200900563

URN: urn:nbn:de:101:1-2023041905593936005619

URL: https://doi.org/10.1002/jssc.200900563

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023041905593936005619

URL: https://d-nb.info/1286587352/34

URL: https://doi.org/10.1002/jssc.200900563

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2004 ACS Award in Separation Science and Technology to William H. Pirkle (2004)

Frank, Hartmut [Verfasser]

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UID:

(DE-101)1303732130

Format: Online-Ressource

ISSN: 1615-9314

In: volume:27

In: number:5‐6

In: year:2004

In: pages:479-479

In: extent:1

In: Journal of separation science, Weinheim : Wiley-VCH, [2000]-, 27, Heft 5‐6 (2004), 479-479 (gesamt 1), 1615-9314

Language: English

DOI: 10.1002/jssc.200490017

URN: urn:nbn:de:101:1-2023092404564947633563

URL: https://doi.org/10.1002/jssc.200490017

URL: https://nbn-resolving.org/urn:nbn:de:101:1-2023092404564947633563

URL: https://d-nb.info/1303732130/34

URL: https://doi.org/10.1002/jssc.200490017

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