Summary
Aldosterone increases the basolateral conductance in target epithelia. The basolateral membrane of tight epithelia contains two different types of K+ conductances (GK), a “resting” and a volume-activated GK. We have studied the early effects (at 4 hours) of 500 nmol/l aldosterone on the basolateral membrane Gk of A6 cells (a Xenopus laevis kidney cell line), after the permeabilization of the apical membrane with amphotericin B. In the presence of a 97 to 3 mmol/l apical to basolateral K+ gradient, the “resting”, inward rectifying GK was similar in control and aldosterone treated cells. In contrast, aldosterone induced a 2-fold increase of the volume-activated quinidine sensitive GK.
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References
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Broillet, M.C., Berger, A. & Horisberger, J.D. Early effects of aldosterone on the basolateral potassium conductance of A6 cells. Pflügers Arch. 424, 91–93 (1993). https://doi.org/10.1007/BF00375106
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DOI: https://doi.org/10.1007/BF00375106