Abstract
Changes in the uterus morphology of mature female rats were studied on the model of pseudopregnancyafter treatment with the progestin 16α,17α-cyclohexanoprogesterone (PR) and the antirpogestins 5α(H)-16α,17α-cyclohexano-4,5-dihydroprogesterone (APR1) and 5β(H)-16α,17α-cyclohexano-4,5-dihydroprogesterone (APR2). The rats were preliminarily estrogenized with 17β-estradiol at a dose of 1 μg/(animal day) for 4 days and then treated with PR at a dose of 0.2 mg/(animal day) for 14 days. The first group was then left without any treatment, whereas APR1 and APR2 were injected at the dose of 0.2 mg/(animal day) for 4 days to the animals of the second and the third groups, respectively. Light and electron microscopy of the uterus preparations demonstrated that the PR action provoked a complete pseudopregnancy picture characterized by the endometrium functionalization and the myometrium hypertrophy. Subsequent treatment with APR1 and APR2 caused the hypertrophy to cease, which had a more pronounced effect in the case of APR2. At the same time, some indications of the endometrium functionalization remained observable after treatment with APR1 and APR2. The specific binding sites for 3H-labeled APR1 and APR2 were absent from the uterus cytosol for the rats gestagenized with PR.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Kamernitskii, A.V. and Levina, I.S., Khim.–Farm. Zh., 1991, no. 10, pp. 4–16.
Levina, I.S., Nikitina, G.V., Kulikova, L.E., and Kamernitskii, A.V., Izv. Ross. Akad. Nauk, Ser. Khim., 1995, no. 3, pp. 564–568.
Kareva, E.N., Kamernitskii, A.V., Levina, I.S., Kirpichnikova, N.V., Kulikova, L.E., and Podval'nyuk, V.V., Byull. Eksp. Biol. Med., 2001, vol. 131, pp. 403–405.
Hurd, C. and Moudgil, V.K., Biochemistry, 1988, vol. 27, pp. 3618–3623.
Smirnov, A.N., Pokrovskaya, E.V., Levina, I.S., Kulikova, L.E., Kamernitskii, A.V., and Shevchenko, V.P., Byull. Eksp. Biol. Med., 2001, vol. 131, pp. 293–296.
MacKenzie, L.W., Cole, W.C., and Garfield, R.E., Acta Physiol. Hungarica, 1985, vol. 65, pp. 461–472.
Garfield, R.E., Basic Mechanisms Controlling Term and Preterm Birth, Chwalish, K. and Garfield, R.E., Eds., Berlin: Springer, 1993, pp. 1–28.
Garfield, R.E., Puri, C.P., and Csapo, A.I., Am. J. Obstetr. Gynecol., 1982, vol. 142, pp. 21–27.
Risek, B., Klier, F.G., Phillips, A., Hahn, D.W., and Gulila, N.B., J. Cell Sci., 1995, vol. 108, pp. 1017–1032.
Kuiper, G.J.M., Carlsson, B., Grandien, K., Enmark, E., Haggblad, J., Nilsson, S., and Gustafsson, J.–A., Endocrinology, 1997, vol. 138, pp. 863–870.
Falkenstein, E. and Meyer, C., Biochem. Biophys. Res. Commun., 1996, vol. 229, pp. 86–89.
Schmidt, B.M., Gerdes, D., Feuring, M., Falkenstein, E., Christ, M., and Wehling, M., Front. Neuroendocrinol., 2000, vol. 21, pp. 57–94.
Shevchenko, V.P., Nagaev, I.Yu., Potapova, A.V., Myasoedov, N.F., Kamernitsky, A.V., Levina, I.S., Kulikova, L.E., and Smirnov, A.N., J. Labelled Compd. Radiopharm., 1998, vol. 41, pp. 919–925.
Smirnov, A.N., Pokrovskaya, E.V., Shevchenko, V.P., Levina, I.S., and Kamernitskii, A.V., Bioorg. Khim., 1999, vol. 25, pp. 774–781.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kamernitsky, A.V., Levina, I.S., Kulikova, L.E. et al. Morphological Changes in Rat Uterus Tissues under the Action of Progestins and Antiprogestins of the Pregna-D"-pentaran Series. Russian Journal of Bioorganic Chemistry 28, 234–241 (2002). https://doi.org/10.1023/A:1015764522454
Issue Date:
DOI: https://doi.org/10.1023/A:1015764522454