Abstract
Mycorrhizal fungus colonization of roots may modify plant metal acquisition and tolerance. In the present study, the contribution of the extraradical mycelium of an arbuscular mycorrhizal (AM) fungus, Glomus mosseae (BEG 107), to the uptake of metal cations (Cu, Zn, Cd and Ni) by cucumber (Cucumis sativus) plants was determined. The influence of the amount of P supplied to the hyphae on the acquisition and partitioning of metal cations in the mycorrhizal plants was also investigated. Pots with three compartments were used to separate root and root-free hyphal growing zones. The shoot concentration of Cd and Ni was decreased in mycorrhizal plants compared to non-mycorrhizal plants. In contrast, shoot Zn and Cu concentrations were increased in mycorrhizal plants. High P supply to hyphae resulted in decreased root Cu concentrations and shoot Cd and Ni concentrations in mycorrhizal plants. These results confirm that some elements required for plant growth (P, Zn, Cu) are taken up by mycorrhizal hyphae and are then transported to the plants. Conversely, Cd and Ni were transported in much smaller amounts by hyphae to the plant, so that arbuscular mycorrhizal fungus colonization could partly protect plants from toxic effects of these elements. Selective uptake and transport of plant essential elements over non-essential elements by AM hyphae, increased growth of mycorrhizal plants, and metal accumulation in the root may all contribute to the successful growth of mycorrhizal plants on metal-rich substrates. These effects are stimulated when hyphae can access sufficient P in soil.
Similar content being viewed by others
References
B D Chen H Shen X L Li G Feng P Christie (2004) ArticleTitleEffects of EDTA application and arbuscular mycorrhizal colonization on growth and zinc uptake by maize (Zea mays L.) in soil experimentally contaminated with zinc Plant Soil 261 219–229 Occurrence Handle1:CAS:528:DC%2BD2cXlvVehtbw%3D
P Christie X Li B Chen (2004) ArticleTitleArbuscular mycorrhiza can depress translocation of zinc to shoots of host plants in soil moderately polluted with zinc Plant Soil 261 209–217 Occurrence Handle10.1023/B:PLSO.0000035542.79345.1b Occurrence Handle1:CAS:528:DC%2BD2cXlvVehtb4%3D
R B Clark S K Zeto (2000) ArticleTitleMineral nutrition by arbuscular mycorrhizal plants J. Plant Nutr. 23 867–902 Occurrence Handle1:CAS:528:DC%2BD3cXksVekt7w%3D Occurrence Handle10.1080/01904160009382068
E George K Häussler D Vetterlein E Gorgus H Marschner (1992) ArticleTitleWater and nutrient translocation by hyphae of Glomus mosseae Can. J. Bot. 70 2130–2137
E George H Marschner I Jakobsen (1995) ArticleTitleRole of arbuscular mycorrhizal fungi in uptake of phosphorus and nitrogen from soil Critic. Rev. Biotechnol. 15 257–270
A Gildon P B Tinker (1983) ArticleTitleInteractions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. II. The effects of infection on uptake of copper New Phytol. 95 263–268 Occurrence Handle1:CAS:528:DyaL2cXlt1Ol
M Giovannetti B Mosse (1980) ArticleTitleAn evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots New Phytol. 84 489–500
Y Guo E George H Marschner (1996) ArticleTitleContribution of arbuscular mycorrhizal fungus to the uptake of cadmium and nickel in bean and maize plants Plant Soil 184 195–205 Occurrence Handle1:CAS:528:DyaK2sXlsVantg%3D%3D
A Johansen I Jakobsen E S Jensen (1992) ArticleTitleHyphal transport of 15N-labelled nitrogen by a vesicular-arbuscular mycorrhizal fungus and its effect on depletion of inorganic soil N New Phytol. 122 281–288 Occurrence Handle1:CAS:528:DyaK3sXns12ltg%3D%3D
E J Joner C Leyval (1997) ArticleTitleUptake of 109Cd by roots and hyphae of Glomus mosseae Trifolium subterraneum mycorrhizal from soil amended with high and low concentrations of cadmium New Phytol. 135 353–360 Occurrence Handle10.1046/j.1469-8137.1997.00633.x Occurrence Handle1:CAS:528:DyaK2sXis12itb8%3D
E J Joner C Leyval (2001) ArticleTitleTime-course of heavy metal uptake in maize and clover as affected by root density and different mycorrhizal inoculation regimes Biol. Fertil. Soils 33 351–357 Occurrence Handle1:CAS:528:DC%2BD3MXjtlGnsb8%3D
K Killham M K Firestone (1983) ArticleTitleVesicular-arbuscular mycorrhizal mediation of grass response to acidic and heavy metal deposition Plant Soil 72 39–48 Occurrence Handle10.1007/BF02185092 Occurrence Handle1:CAS:528:DyaL3sXltVSmsb0%3D
A Kloke (1980) ArticleTitleOrientierungsdaten für tolerierbare Gesamtgehalts einiger Elemente in Kulturböden Mitt. VDLUFA H.1 9–11
R E Koske J N Gemma (1989) ArticleTitleA modified procedure for staining roots to detect VA mycorrhizas Mycol. Res. 92 486–505
S K Kothari H Marschner V Römheld (1991) ArticleTitleContribution of VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcareous soil Plant Soil 131 177–185 Occurrence Handle10.1007/BF00009447 Occurrence Handle1:CAS:528:DyaK3MXht1SrsLc%3D
X-L Li H Marschner E George (1991) ArticleTitleAcquisition of phosphorus and copper by VA-mycorrhizal hyphae and root-to-shoot transport in white clover Plant Soil 136 49–57 Occurrence Handle1:CAS:528:DyaK3MXmsFGis7c%3D
X-L Li E George H Marschner J-L Zhang (1997) ArticleTitlePhosphorus acquisition from compacted soil by hyphae of a mycorrhizal fungus associated with red clover (Trifolium pratense) Can. J. Bot. 75 723–729 Occurrence Handle1:CAS:528:DyaK2sXltVOltrY%3D
W L Lindsay W A Norvell (1978) ArticleTitleDevelopment of a DTPA soil test for zinc, iron, manganese, and copper Soil Sci. Soc. Am. J. 42 421–443 Occurrence Handle1:CAS:528:DyaE1cXltVKntLs%3D Occurrence Handle10.2136/sssaj1978.03615995004200030009x
J Murphy J P Riley (1962) ArticleTitleA modified single solution method for the determination of phosphate in natural waters Anal. Chem. Acta 27 31–36 Occurrence Handle10.1016/S0003-2670(00)88444-5 Occurrence Handle1:CAS:528:DyaF38XksVyntr8%3D
E Neumann E George (2004) ArticleTitleColonisation with the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) enhanced phosphorus uptake from dry soil in Sorghum bicolor (L.) Plant Soil 261 245–255 Occurrence Handle10.1023/B:PLSO.0000035573.94425.60 Occurrence Handle1:CAS:528:DC%2BD2cXlvVeht7o%3D
E Neumann E George (2005) ArticleTitleDoes the presence of arbuscular mycorrhizal fungi on growth and nutrient uptake of a wild-type tomato cultivar and a mycorrhiza-defective mutant, cultivated with roots sharing the same soil volume? New Phytol. 166 601–609 Occurrence Handle10.1111/j.1469-8137.2005.01351.x Occurrence Handle1:CAS:528:DC%2BD2MXktlWqsb0%3D Occurrence Handle15819922
T E Pawlowska I Charvat (2004) ArticleTitleHeavy-metal stress and developmental patterns of arbuscular mycorrhizal fungi Appl. Environ. Microbiol. 70 6643–6649 Occurrence Handle10.1128/AEM.70.11.6643-6649.2004 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSju7zM Occurrence Handle15528529
J N Pearson I Jakobsen (1993) ArticleTitleThe relative contribution of hyphae and roots to phosphorus uptake by arbuscular mycorrhizal plants, measured by dual labelling with 32P and 33P New Phytol. 124 489–494 Occurrence Handle1:CAS:528:DyaK2cXhtleit7s%3D
H Schüepp B Dehn H Sticher (1987) ArticleTitleInteraktionen zwischen VA-Mykorrhiza und Schwermetallbelastungen Angew. Bot. 61 85–96
S E Smith F A Smith I Jakobsen (2004) ArticleTitleFunctional diversity in arbuscular mycorrhizal (AM) symbioses: The contribution of the mycorrhizal P uptake pathway is not correlated with mycorrhizal responses in growth or total P uptake New Phytol. 162 511–524 Occurrence Handle10.1111/j.1469-8137.2004.01039.x
M Z Solaiman T Ezawa T Kojima M Saito (1999) ArticleTitlePolyphosphates in intraradical and extraradical hyphae of an arbuscular mycorrhizal fungus, Gigaspora margarita Appl. Environ. Microbiol. 65 5604–5606 Occurrence Handle1:CAS:528:DyaK1MXnvVGju7k%3D Occurrence Handle10584026
L Tudoreanu C J C Phillips (2004) ArticleTitleModeling cadmium uptake and accumulation in plants Adv. Agron. 84 121–157 Occurrence Handle1:CAS:528:DC%2BD2MXnvVynsA%3D%3D
I Weissenhorn C Leyval (1995) ArticleTitleRoot colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture Plant Soil 175 233–238 Occurrence Handle10.1007/BF00011359 Occurrence Handle1:CAS:528:DyaK2MXotFOltbo%3D
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, Y.J., George, E. Contribution of Mycorrhizal Hyphae to the Uptake of Metal Cations by Cucumber Plants at Two Levels of Phosphorus Supply. Plant Soil 278, 361–370 (2005). https://doi.org/10.1007/s11104-005-0373-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11104-005-0373-1