Summary
The culture supernatants of 126 bacterial strains isolated during screening for hydrophobic cell surfaces, were tested for the production of emulsifying agents. Forty-eight strains were found to produce effective emulsion-stabilizing substances during growth on glucose. The most effective emulsifying agents were isolated and could be divided into two chemical groups. The first group was separated from the isolated extracts by the use of thin-layer chromatography and detected as ninhydrin-negative, 4,4'-tetramethyldiamino-diphenylmethane-positive spots. The amino acid composition indicated surfactin and iturin, produced by one Bacillus species, and viscosin, produced by a Pseudomonas species. The second group was identified as polymeric substances. The chemical characterization of five polymers showed polysaccharides that were able to stabilize emulsions. From these the neutral and charged monosaccharides were determined qualitatively. The constituents of the five isolated polysaccharides were: strain 5, glucose, strain 17, rhamnose, glucose, glucuronic acid; strain 33, rhamnose, galactose, glucose. glucuronic acid; strain 113, fucose, galactose, glucose, galacturonic acid, glucosamine; strain 259, one unknown compound, rhamnose, galactose, glucuronic acid.
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References
Akit J, Zajic JE (1982) Surface properties and surfactant production of Corynebacterium fascians at different hydrophobicities. Dev Ind Microbiol 23:445–457
Arx E von, Faupel M, Brugger M (1976) Das 4,4'-Tetramethyldiaminodiphenyl methan Reagens (TDM). Eine Modifikation der Chlor-o-Tolidin Färbereaktion für die Dünnschichtchromatographie. J Chromatogr 120:224–228
Bar-Ness R, Avrahamy N, Matsuyama T, Rosenberg M (1988) Increased cell surface hydrophobicity of a Serratia marcescens NS 38 mutant lacking wetting activity. J Bacteriol 170:4361–4364
Besson F, Peypoux F, Michel G, Delcambe L (1978) Identification of antibiotics of iturin group in various strains of Bacillus subtilis. J Antibiot (Tokyo) 31:283–288
Christensen BE, Kjosbakken J, Smidrod O (1985) Partial chemical an physical characterization of two extracellular polysaccharides produced by marine, periphytic Pseudomonas sp. NCMB 2021. Appl Environ Microbiol 50:837–845
Cooper DG, Zajic JE (1980) Surface-active compounds from microorganisms. Adv Appl Microbiol 26:229–253
Costerton JW, Irwin RT, Cheng K-J (1981) The bacterial glycocalyx in nature and disease. Ann Rev Microbiol 35:299–324
Erne AM, Werner RG, Reifenrath R (1984) Inhibition of bacterial adhesion by an artificial surfactant. FEMS Microbiol Lett 23:205–209
Fattom A, Shilo M (1985) Production of emulcyan by Phormidium J-1: its activity and function. FEMS Microbiol Ecol 31:3–9
Floodgate GD (1978) The formation of oil emulsifying agents in hydrocarbonclastic bacteria. In: Loutit MW, Miles JAR (eds) Microbial ecology. Springer, Berlin, Heidelberg, New York, pp 82–85
Haferburg D, Hommel R, Claus R, Kleber H-P (1986) Extracellular microbial lipids as biosurfactants. Adv Biochem Eng/Biotechnol 33:53–93
Humphrey BA, Marshall KC (1984) The triggering effect of surfaces an surfactants on heat output, oxygen consumption and size reduction of a starving marine Vibrio. Arch Microbiol 140:166–170
Kakinuma A, Arima K (1969) Surfactin, a novel peptidelipid surfactant produced by Bacillus subtilis. Ann Rep Takeda Res Lab 28:139–193
Kickhöfen B, Warth R (1976) Eine Trennkammer für die Hochspannungselektrophorese nach dem Michl'schen Prinzip. J Chromatogr 33:559–560
Kosaric N, Gray NCC, Cairns WL (1983) Microbial emulsifiers and de-emulsifiers. In: Rehm H-J, Reed G (eds) Biotechnology, vol 3. Verlag Chemie, Weinheim, pp 575–592
Leisinger T, Margraff R (1979) Secondary metabolites of the fluorescent pseudomonads. Microbiol Rev 43:422–442
McEldowney S, Fletcher M (1986) Variability of the influence of physicochemical factors affecting bacterial adhesion to polystyrene substrata. Appl Environ Microbiol 52:57–62
Neu TR, Poralla K (1987) Screening for bacteria with hydrophobic surfaces — a strategy for isolating surface-active molecules. In: Neijssel OM, Meer RR van der, Luyben KCAM (eds) Proceedings of the 4th European Congress on Biotechnology, Amsterdam, June 14–19, 1987, vol 3. Elsevier, Amsterdam, pp 397–399
Neu TR, Poralla K (1988) An amphiphilic polysaccharide from an adhesive Rhodococcus strain. FEMS Microbiol Lett 49:389–392
Neu TR, Härtner T, Poralla K (1990) Surface active properties of viscosin — a peptidolipid antibiotic. Appl Microbiol Biotechnol 32:518–520
Parkinson M (1985) Bio-surfactants. Biotechnol Adv 3:65–83
Paul JH, Jeffrey WH (1985) The effect of surfactants on the attachment of estuarine and marine bacteria to surfaces. Can J Microbiol 31:224–228
Peypoux F, Guinand M, Michel G, Delcambe L, Das BC, Lederer E (1978) Structure of iturine A, a peptidolipid antibiotic from Bacillus subtilis. Biochemistry 17:3992–3996
Rosenberg E (1986) Microbial surfactants. CRC Crit Rev Biottechnol 3:109–132
Rosenberg E, Perry A, Gibson DT, Gutnick DL (1979a) Emulsifier of Arthrobacter RAG-1: specificity of hydrocarbon substrate. Appl Environ Microbiol 37:409–413
Rosenberg E, Zuckerberg A, Rubinovitz C, Gutnick DL (1979b) Emulsifier of Arthrobacter RAG-1: isolation and emulsifying properties. Appl Environ Microbiol 37:402–408
Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity. FEMS Microbiol Lett 9:29–33
Rosenberg E, Kaplan N, Pines O, Rosenberg M, Gutnick D (1983) Capsular polysaccharides interfere with adherence of Acinetobacter calcoaceticus to hydrocarbon. FEMS Microbiol Lett 17:157–160
Sandford PA, Baird J (1983) Industrial utilization of polysaccharides. In: Aspinall GO (ed) The polysaccharides, vol 2. Academic Press, New York, pp 411–490
Savage DC, Fletcher M (1985) Bacterial adhesion. Plenum Press, New York
Sutherland IW, Ellwood DC (1979) Microbial exopolysaccharides — industrial polymers of current and future potential. In: Bull AT, Ellwood DC, Ratledge C (eds) Microbial technology: current state future prospects. 29th Symposium of the Society for General Microbiology. University Press, Cambridge, pp 107–150
Symes KC (1982) Lipophilic polysaccharides. Carbohydr Polymers 2:276–281
Trevelyan WE, Procter DP, Harrison JS (1950) Detection of sugars on paper chromatograms. Nature 166:444–445
Zajic JE, Panchal CJ (1976) Bio-emulsifiers. CRC Crit Rev Microbiol 5:39–66
Zajic JE, Seffens W (1984) Biosurfactants. CRC Crit Rev Biotechnol 1:86–107
ZoBell CE (1947) Bacterial release of oil from oil bearing materials. Oil Gas J 46:62
Zuckerberg A, Diver A, Peeri Z, Gutnick DL, Rosenberg E (1979) Emulsifier of Arthrobacter RAG-1: chemical and physical properties. Appl Environ Microbiol 37:414–420
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Neu, T.R., Poralla, K. Emulsifying agents from bacteria isolated during screening for cells with hydrophobic surfaces. Appl Microbiol Biotechnol 32, 521–525 (1990). https://doi.org/10.1007/BF00173721
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DOI: https://doi.org/10.1007/BF00173721