Mycobacterium tuberculosis infection modulates adipose tissue biology
Fig 1
Mtb infects human and mouse adipocytes in vitro and expresses stress-related genes.
(A-C) Immunofluorescence staining and quantification of extra- and intracellular Mtb-GFP at multiplicity of infection (MOI) of 20 after 24 h in (A) SGBS human cell strain; (B) primary adipocytes from human subcutaneous fat; (C) 3T3-L1 mouse cell line. Blue: DAPI. Original magnification 1,000×. Data are representative of two independent experiments (triplicates). (D-F) log10 Mtb CFUs at indicated time points post-infection with MOI = 5 in the presence or absence of amikacin in (D) SGBS human cell strain, (E) 3T3-L1 mouse cell line, (F) primary adipocytes from human subcutaneous fat; data are representative of two to four independent experiments. (G-H) Expression of Mtb genes dosR, hspX and lat in (G) SGBS human cell strain and (H) 3T3-L1 mouse cell line. Cells were infected with MOI = 5. Steady state transcript levels were normalized to Mtb sigA expression. Means of duplicates from four to six independent experiments are shown. Abbreviations: E, extracellular; I, intracelular; SGBS, Simpson-Golabi-Behmel syndrome.