The Novel Immunosuppressive Protein Kinase C Inhibitor Sotrastaurin Has No Pro-Viral Effects on the Replication Cycle of Hepatitis B or C Virus
Figure 1
Differential effect of sotrastaurin on HCV RNA replication and virus infection.
(A) Replication of HCV in the presence of sotrastaurin at 4, 24, 48 and 72 h post transfection. (B) Inoculation of naïve Huh-7.5 cells using culture fluid from the cells shown in Fig. 1A. After 4 h, cells were supplemented with fresh medium without sotrastaurin. (C+D) Treatment of Huh-7.5 cells harboring a subgenomic HCV replicon (SG-JFH1-Luc) with increasing concentrations of (C) sotrastaurin or (D) BIM-I. The DMSO control corresponds to the DMSO concentration present with the highest drug concentration tested. The luciferase assay was performed 48 hours post transfection. (E) Huh-7.5 cells were treated with increasing amounts of sotrastaurin. After 48 h cell were stained with a live/dead cell stain kit and analyzed by FACS. (F) Percentage of BrdU-positive cells after 48 hours. (G) Luciferase activity as an aggregate measure of viability and proliferation in Huh-7.5 cells stably expressing luciferase under a CMV-promoter treated with sotrastaurin for 72 h.