Epstein-Barr virus (EBV) activates NKL homeobox gene HLX in DLBCL
Fig 1
Characterization of EBV-positive/negative DOHH-2 subclones.
(A) FISH analysis of EBV in isolated DOHH-2 subclones. The EBV-probe was labelled in green and the nuclei were counterstained with DAPI in blue. (B) PCR analysis of EBV-DNA in isolated subclones. Both procedures confirmed the presence and absence of EBV and thus the purity of the isolated DOHH-2 subclones. (C) RT-PCR analysis of selected EBV-encoded genes showing activity for LMP1, LMP2A, EBNA1, EBNA2, EBNA3A and EBNA3C in EBV-positive DOHH-2 cells. The gene YY1 served as positive control, NTC: no template control. (D) RQ-PCR analysis of CD19, CD21, CD38, CD86 and CD138 in EBV-negative and EBV-positive DOHH-2 subclones. (E) Immuno-fluorescence microscopy analysis was performed for IgG immunoglobulins (green) in EBV-negative (left) and EBV-positive (right) DOHH-2 cells. The nuclei were counterstained by DAPI (blue). (F) Microscopical analysis of living EBV-negative and EBV-positive DOHH-2 subclones in culture. Note that cell aggregates are only visible in EBV-positive DOHH-2 cultures. (G) Quantitative analysis via live-cell imaging analysis of EBV-negative and EBV-positive DOHH-2 subclones indicated no difference in their rates of proliferation.