Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei
Fig 1
Experimental setup and differentiation proteome.
(A) Schemata of the differentiation process and the experimental time points. Long slender bloodstream forms (LS) were differentiated to short stumpy forms (SS) followed by differentiation to insect form trypanosomes (PF). Parasites were harvested and analyzed by label-free quantitative mass spectrometry at different time points during the differentiation process as indicated. (B) The heat map encompassing 4270 z-scored LFQ quantified protein groups illustrates the major proteome remodeling. Significant expression differences can be compared between individual time points during the differentiation process. (C) Principle component analysis shows high reproducibility of replicates (r = 0.92–0.99) and consecutive progression of the differentiation process that can be described by the first two components PC1 and PC2.