In Vivo Imaging Enables High Resolution Preclinical Trials on Patients’ Leukemia Cells Growing in Mice
Figure 2
In vivo imaging of patient-derived ALL in mice. a
Kinetics of leukemic growth; 5×104 ALL-177 cells per mouse were injected into a group of 5 mice which were imaged repeatedly over time; a single representative mouse is shown; units in rainbow color scales are photons per second per cm2 per steradian (photons s−1 cm2−1 sr−1); b Dose-response of leukemic growth; 1×107–3×104 serially diluted ALL-177 cells were injected into groups of 5 mice which were imaged 8 weeks after injection; a single representative mouse is shown for each group; c, dGood correlation of in vivo imaging to post mortem readout parameters; 12 mice were injected with 105 ALL-50 cells/mouse; each week, 2 mice were imaged and sacrificed; organs were collected and cell suspensions prepared from half the organ and 1 femur and were analyzed by flow cytometry for expression of GFP/human CD38; the other half of the organ and the second femur were analyzed by immunohistochemistry for expression of terminal desoxynucleotidyl transferase (Tdt; arrows indicate leukemia cells); rare, +, ++,+++indicate a rough quantification of the number of leukemic cells per field; c shows 1 representative image per week and post mortem analysis of bone marrow; in all mice, mid-abdominal signals are unspecific. d correlates results from imaging and FACs analysis in each mouse; correlation coefficient 0,86; Supplemental Figure 6 shows data on spleens.