Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis
Figure 2
Pulse-field gel electrophoresis (PFGE) confirmation of the potential PiraGV-K DNA.
HMW DNA embedded agarose plugs of P. rapae confirmed by PFGE, wherein the plugs were partially digested by an enzyme mixture following pre-electrophoresis. ‘M’ represents PFG lambda marker (NEB) and lanes 1–5 depict EcoRI digested DNA molds. A potential PiraGV-K DNA band was seen approximately at 125 kb after PFGE of enzyme digested DNA. PFGE conditions included 1% pulsed field certified agarose gel, a pulse time between 0.1–40 sec for up to 16 hrs and a voltage of 6 V/cm to check for partially digested plugs.