True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells
Figure 2
Customized TALENs targeting gfp.
(a) Four basic modular amino acid repeats with their repeat variable diresidues indicated by the standard single letter codes shown in the colored boxes. The preferred nucleotide recognized by the individual repeat is shown below the repeat. (b) TALENs and their target sequences in the gfp. DNA binding specificity was achieved by the arrays of repeats assembled according to the target sequence. The nucleotide sequence shown in red represents the left and the one shown in blue the right-hand TALEN binding site. Lower case letters represent the region where FokI domains are expected to dimerize and introduce DSBs.