Joint Binding of OTX2 and MYC in Promotor Regions Is Associated with High Gene Expression in Medulloblastoma
Figure 3
OTX2 motifs enriched at OTX2 peak centers, but not at TSSs.
A. The percentage of the OTX2-binding motifs (TAATCC, TAAGCC and TAATCT) per 50 bp were plotted in regards to the OTX2-binding peak center. For all three motifs, enrichment at the center of OTX2-binding peaks is observed, although for TAATCC this was more apparent in repeat masked data (red) compared to unmasked data (blue). Similar results were obtained for the E-box sequences in the MYC experiment. B. The presence of OTX2- and MYC-binding motifs was scored per 50 bp for all promoter regions in the genome. While E-box sequences display a higher frequency near TSSs in the genome, TAATCC, TAAGCC and TAATCT do not. They all show a clear depletion near TSSs, which is most clear in data without repeat mask (blue lines) as compared to data with repeat mask (red lines).