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Salt Tolerance Mechanism and Species Identification of the Plant Rhizosphere Bacterium JYZ-SD2

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Abstract

A salt-tolerant microbe strain JYZ-SD2 was investigated to develop biological soil amendments to stimulate salix growth and acclimation in costal salt-affected soils. The salt tolerance mechanism of strain JYZ-SD2 was investigated by detecting the salt-tolerant growth characteristics, biofilm formation, ion distribution, secondary metabolites, and zymogram profiling. The strain was identified by physiological and biochemical characteristics (Biolog), 16S rDNA sequencing, and cry1/7/9 gene expressing. With increasing of NaCl concentration, strain JYZ-SD2 adapted to the increased osmotic pressure by prolonging the retardation period, slowing down the growth rate of the logarithmic phase, increasing spo0A gene expression, increasing biofilm formation, reducing Na+ uptake, and changing the expression of metabolites and intracellular soluble proteins. The results showed that strain JYZ-SD2 could be assigned to Bacillus cereus.

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Acknowledgements

This study was supported by the National Key Research and Development Program of China (Grant No. 2017YFD0600100) and the Priority Academic Development Program of Jiangsu Universities (PAPD). I am grateful to Dr. Prof. Eric C. Brevik, Dickinson State University, for his kind improving this paper.

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  1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China

    Tian-yu Wu, Xiao-Qin Wu, Xiu-qian Xu, Wei-liang Kong & Fei Wu

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  1. Tian-yu Wu
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Wu, Ty., Wu, XQ., Xu, Xq. et al. Salt Tolerance Mechanism and Species Identification of the Plant Rhizosphere Bacterium JYZ-SD2. Curr Microbiol 77, 388–395 (2020). https://doi.org/10.1007/s00284-019-01835-0

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