A Histone Methyltransferase Modulates Antigenic Variation in African Trypanosomes
Figure 4
ΔDOT1B Double-Expressers Are Stable Switching Intermediates
(A) A population of ΔDOT1B Class II cells brightly stained with anti-VSG221 and anti-VSG13 (square) was sorted and subcloned by limiting dilution either in the presence of 100 μg/ml G418 (60 d) or in the absence of any drug (30 d).
(B) Six clones grown in the presence of G418 were submitted to FACS analysis every 8 d to follow the proportion of cells that exclusively express VSG13.
(C) After 5 and 30 d without any antibiotics, VSG221 and VSG13 expression was measured by FACS. The profile of one representative clone is shown.
(D) After 30 d without antibiotic, cells were diluted in 25 or 75 μg/ml G418, and cell growth was followed for 7 d.