ComEA Is Essential for the Transfer of External DNA into the Periplasm in Naturally Transformable Vibrio cholerae Cells
Figure 4
In vivo validation of ComEA-DNA interaction sites.
Strains carrying comEA-mCherry or variants thereof on the chromosome were tested for uniform periplasmic localization of the fusion protein and foci formation after the addition of external transforming DNA (A), for DNA uptake (B) as described for Fig. 2, or for natural transformation (C). The transformation frequencies shown on the Y-axis are averaged from at least three independent replicates (± SD). <d.l., below detection limit (on average 3.4×10−8 ± SD of 1.1×10−8). Statistically significant differences were determined using Student's t-test on log-transformed values. **P<0.01.