Human Anti-Plague Monoclonal Antibodies Protect Mice from Yersinia pestis in a Bubonic Plague Model
Figure 4
Specific binding of the human anti-F1 IgG1 to the Y. pestis cell surface was detected by a flow cytometry assay.
A mouse anti-B mallei IgG1was used (10 µg/ml) as an isotype mAb control, and the mouse anti-F1 (F1-04-A-G1, F1m) mAbs was used (10 µg/ml) as a positive IgG1 control. Y. pestis grown at 26°C (top panel) was used as an anti-F1 mAb negative control. E.coli grown at 37°C was used as a specificity control for anti-F1 and anti-V IgGs.