Biophysical and biochemical studies support TP0094 as a phosphotransacetylase in an acetogenic energy-conservation pathway in Treponema pallidum
Fig 4
Structure-based alignment of TP0094 and MtPta.
The sequences of TP0094 and MtPta, as found in their respective PDB files (Acc. numbers 8FIR and 1QZT, chain C, respectively) are shown as aligned by PROMALS3D [35]. The numbering is from TP0094, and secondary structural elements, as defined by those found in the TP0094 structure, are shown above the alignment; α-helices are coils and β-strands are arrows. Residues highlighted in red are identical, and those outlined in blue have chemical similarity. Orange triangles mark residues that putatively contact acetyl phosphate, blue squares mark putative CoA-contacts, and red stars mark the three putatively catalytic residues. The boundaries of Domains I (purple) and II (green) are shown with lines. The figure was generated using ESPript 3.0 (https://espript.ibcp.fr [36]).