Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei
Fig 4
Analysis of early differentiation events from short stumpy to procyclic form.
(A) Cumulative growth curve after induction of in vitro differentiation. After one population doubling ∆DOT1B trypanosomes undergo growth arrest and die after several days. (B) Detection of procyclin on the surface of differentiating trypanosomes by flow cytometry. Increasing relative fluorescence correlates with the amount of surface exposed procyclin during the course of differentiation. Procyclin expression is indistinguishable in wild-type and ∆DOT1B populations. (C) BrdU incorporation of differentiating trypanosomes. When differentiating cells re-enter the cell cycle both cell lines incorporate BrdU into freshly replicated DNA. Addition of BrdU for different time periods during differentiation demonstrates that the number of actively replicating cells decreases in DOT1B-deficient cells. The statistical analysis with an unpaired t-test shows significance between the data sets marked with bars and asterisks (* p-value <0.05, ** p-value <0.01).