The Indoleamine-2,3-Dioxygenase (IDO) Inhibitor 1-Methyl-D-tryptophan Upregulates IDO1 in Human Cancer Cells
Figure 5
Upregulation of IDO1 mRNA by 1-D-MT in SKOV-3 cells.
(A) Left panel: mRNA expression of IDO1, IDO2 and TDO in SKOV-3 cells after treatment with 1 mM 1-D-MT, analyzed after 24 h by qRT-PCR. Right panel: Western Blot analysis of IDO1 expression in SKOV-3 cells performed after 48 h 1 mM 1-D-MT. GAPDH served as loading control. (B) IDO1 mRNA expression in response to increasing concentrations of 1-D-MT measured after 24 h by qRT-PCR. (C) Time course analysis of IDO1 mRNA induction by 1 mM 1-D-MT, analyzed by qRT-PCR. Experiments were performed in triplicate. Data are mean ± SEM. * (p<0.05).