In Vivo Imaging Enables High Resolution Preclinical Trials on Patients’ Leukemia Cells Growing in Mice
Figure 5
Precise quantification of individual treatment effects and monitoring of distinct clinical stages. a
Imaging visualizes treatment-induced cell loss and regrowth; 105 ALL-50 cells/mouse were injected into 10 mice which received a single intraperitoneal dose of Etoposid (VP-16; 50 mg/kg) in week 6 after tumor cell injection, except the control mouse which was treated with PBS. Animals were imaged before treatment (pre-treatment) and 4 and 11 days after treatment; shown is one representative mouse; all mice are shown in Supplemental Figure 11; b, cImaging visualizes different sensitivities of individual samples towards treatment; ALL-199 (1×104 cells/mouse) or ALL-4S (5×104 cells/mouse) were injected into 16 mice; mice were randomized in week 4 into one control (n = 4) and two experimental groups (n = 6 each). Control mice received buffer injection, while the other groups were treated once intraperitoneally with either Etoposid (VP-16; 50 mg/kg) or cyclophosphamide (Cyclo; 150 mg/kg) as indicated. Mice were imaged directly before and 4 days after treatment; shown are 3 representative mice of each treatment group before and 4 days after treatment (b); shown is the result of quantification of the images; each line represents a single mouse (c); * P<0.05, *** P<0.001, Mann-Whitney Rank Sum Test; d Imaging visualizes disease stages known from patients; 15 mice were injected with 1×106 ALL-199 cells/mouse and treated once intraperitoneally with Etoposid (VP-16; 50 mg/kg) in week 4; after treatment, mice were imaged three times per week; shown is the mean +/− SEM of image quantifications.