Gastric Antimicrobial Peptides Fail to Eradicate Helicobacter pylori Infection Due to Selective Induction and Resistance
Figure 6
Radial diffusion assay, electron microscopy and binding experiments.
In the RDA all four H. pylori strains displayed inhibition zones after incubation with 20 µg HBD3 or LL37, but not with HBD1, -4 or elafin. With HBD2 marginal growth inhibition was observed for two strains. Investigated with electron microscopy, cells of H. pylori KaE1 showed more pronounced disintegration with 200 µg/ml HBD3 than with 200µg/ml HBD2 (B). In flow cytometric experiments with biotinylated antibodies, HBD2 and LL37 bound to E. coli ATCC 25922 and Helicobacter pylori (C).