Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography
Fig 6
Flow cytometry and WB analysis targeting the exosome marker CD9 on isolated vesicles.
CD9 positive vesicles were detected by flow cytometry. Median fluorescence intensity (MFI) was reported as a signal to noise (S/N) ratio to isotype control in EVs isolated from E10 (A), BxPC3 (B) and H3 (C) cells (n = 3). The presence of CD9 was also analyzed by WB, which was detected in vesicles from E10 (D) and BxPC3 cells (E) (n = 3).