The genetic basis for adaptation of model-designed syntrophic co-cultures
Fig 7
The top panel depicts the dynamics of high multiplicity duplications in two transport complexes throughout the course of ALE #5 of a ΔhisD & ΔgdhAΔgltB co-culture. A small region containing the abgT symporter of p-aminobenzoyl glutamate was duplicated early in the evolution, but later duplications in a region containing gltJ, along with the rest of the genes comprising the GltIKJL L-glutamate/L-aspartate ABC uptake system, became more prevalent. The bottom panel depicts the course of ALE #11, a ΔhisD & ΔgltAΔprpC co-culture that initially showed a broad ~1 Mbp duplication. By the end of the evolution either a nested duplication emerged in a small genome region containing hisJ, along with the rest of the HisJMPQ L-histidine ABC uptake system, or a significant subpopulation emerged containing this duplication.