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The Extracellular Matrix Protein SC1/Hevin Localizes to Multivesicular Bodies in Bergmann Glial Fibers in the Adult Rat Cerebellum

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Abstract

SC1 is an extracellular matrix molecule prominent in the mammalian brain. In the cerebellum, SC1 localizes to Bergmann glial cells and perisynaptic glial processes that envelop synapses in the molecular layer. In the present study, confocal microscopy revealed a punctate distribution of SC1 along Bergmann glial fibers that colocalized with the intermediate filament GFAP when fibers were viewed in cross-section. Immunoelectron microscopy showed that the punctate SC1 pattern corresponded to the localization of SC1 in multivesicular bodies situated within Bergmann glial fibers. The pattern of SC1 localization was not disrupted following hyperthermia or pilocarpine-induced status epilepticus. The present study suggests that SC1 protein may reach its destination in perisynaptic glial processes and glial endfeet by transport along Bergmann glial fibers in multivesicular bodies and that this process is preserved following stress.

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Acknowledgments

We thank Professor James W. Gurd and Crystal Dykstra for the methodology of the rat lithium-pilocarpine seizure model. This work was supported by grants to I.B. from the National Science and Engineering Research Council of Canada who also holds a Canada Research Chair.

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  1. Starlee Lively

    Present address: Toronto Western Research Institute, University Health Network, Toronto, ON, Canada

Authors and Affiliations

  1. Center for the Neurobiology of Stress, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON, M1C 1A4, Canada

    Starlee Lively & Ian R. Brown

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  2. Ian R. Brown
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Correspondence to Ian R. Brown.

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Lively, S., Brown, I.R. The Extracellular Matrix Protein SC1/Hevin Localizes to Multivesicular Bodies in Bergmann Glial Fibers in the Adult Rat Cerebellum. Neurochem Res 35, 315–322 (2010). https://doi.org/10.1007/s11064-009-0057-y

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  • DOI: https://doi.org/10.1007/s11064-009-0057-y

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