578559471  

Standardization and quality assurance in fluorescence measurements II : bioanalytical and biomedical Applications / vol. ed.: Ute Resch-Genger

Englisch

Berlin, Heidelberg : Springer-Verlag Berlin Heidelberg, 2008

Online-Ressource : Ill., graph. Darst.

Description based upon print version of record

Print version: Standardization and Quality Assurance in Fluorescence Measurements II

978-3-540-70571-0

Weitere Ausgaben: 978-3-540-70570-3 (Druckausgabe)

DOI: 10.1007/978-3-540-70571-0

Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer's View; Characterization and Calibration in Wide Field and Sectioned Fluorescence Microscopy SIPcharts; Quantitative Fluorescence Microscopy: Considerations and Controls; Comparability of Fluorescence Microscopy Data and Need for Instrument Characterization of Spectral Scanning Microscopes; Fluorescence Lifetime Imaging Microscopy: Quality Assessment and Standards; State of the Art and Novel Trends in Fluorescence Correlation Spectroscopy

Single Molecule Spectroscopy: Instrumentation and Multiparameter DetectionDNA Microarrays: Applications, Future Trends, and the Need for Standardization; Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization; Microarray Technology: Unresolved Issues and Future Challenges from a Regulatory Perspective; Protein Arrays and Fluorescence Detection: Applications and Limitations; Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations; Flow Cytometry Quality Assurance; Approaches to Quantitation in Flow Cytometry

Immunoassays: Basic Concepts, Physical Chemistry and ValidationTime-Resolved Fluorometric Immunoassays; Instrumentation, Applications, Unresolved Issues and Future Trends; Particle-Based Assays: Applications and Unresolved Issues; Advances in Fluorescence Enzyme Detection Methods; Quantitative Real-Time PCR: Fluorescent Probe Options and Issues; Cellular Bioimaging in Fluorescent Cancer Biomarker Evaluation: Validation, Technologies and Standards Development; Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues

In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization

*Fluoreszenzanalyse

*Analytical biochemistry ; Biochemistry ; Medicine ; Weights and measures ; Chemistry ; Surfaces (Physics)

Klassifikation der Library of Congress: QD96.F56

Dewey Dezimal-Klassifikation: 543.56;

Regensburger Verbund-Klassifikation: VG 8750: V Chemie und Pharmazie / VG Analytische Chemie / VG 5000-VG 9909 Analytische Chemie allgemein / VG 8750-VG 8759 Fluoreszenzanalyse; Atomfluoreszenzspektroskopie; Lumineszenzanalyse; Fluorimetrie / Allgemeines

Analytical chemists and materials scientists will find this a useful addition to their armory. The contributors have sought to highlight the present state of affairs in the validation and quality assurance of fluorescence measurements, as well as the need for future standards. Methods included range from steady-state fluorometry and microfluorometry, microscopy, and micro-array technology, to time-resolved fluorescence and fluorescence depolarization imaging techniques.

The validation and standardization of fluorescence methods is still in its infancy as compared to other prominent analytical and bioanalytical methods. Appropriate quality assurance standards are however a prerequisite for applications in highly regulated fields such as medical diagnostics, drug development, or food analysis. For the first time, a team of recognized international experts has documented the present status of quality assurance in fluorescence measurements, and outlines concepts for establishing standards in this field. This second of two volumes covers applications of fluorescence for bioanalysis and biomedical diagnostics.

 

Online-Ausg.

2008

Electronic reproduction; Available via World Wide Web

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http://dx.doi.org/10.1007/978-3-540-70571-0 [E-books (Springer)]