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Cover -- Copyright -- Contents -- Preface -- Chapter 1: The perfect slime - and the "dark matter" of biofilms -- Abstract -- 1.1 What is 'Perfect'? -- 1.2 The Matrix: Basis for the Emergent Properties of Biofilms -- 1.3 The "Dark Matter of Biofilms" -- 1.3.1 What influences EPS production and how can it be managed? -- 1.3.2 Where and by which mechanisms are hydrophobic substances sorbed in the matrix? -- 1.3.3 What are the mechanisms behind water retention? -- 1.3.4 Can the permeability of biofouling layer be engineered in membrane technology? -- 1.3.5 Which are the interactions of the various EPS components? -- 1.3.6 What is the function of complex EPS components? -- 1.3.7 Which EPS components contribute to matrix stability and how can we predict it? -- 1.4 Chaos and Function - Self-Organization -- Acknowledgements -- References -- Chapter 2: EPS - a complex mixture -- Abstract -- 2.1 Introduction -- 2.2 Microbial Polysaccharide Composition -- 2.3 Polysaccharide Structure -- 2.4 Physical Properties -- 2.5 Polysaccharide Interactions -- References -- Chapter 3: The extracellular matrix - an intractable part of biofilm systems -- Abstract -- 3.1 Introduction -- 3.2 Challenges -- 3.3 Biofilm Matrix Analysis -- 3.4 Biofilm Matrix Constituents -- 3.4.1 Polysaccharides -- 3.4.2 Proteins -- 3.4.3 Amyloids -- 3.4.4 Extracellular nucleic acids -- 3.4.5 Amphiphilic compounds -- 3.4.6 Membrane vesicles -- 3.4.7 Refractory compounds -- 3.5 Bacterial Extracellular Biology -- 3.6 Biofilm Matrix Functionality -- 3.6.1 Architecture -- 3.6.2 Protection -- 3.6.3 Cryo- and Osmo-protection -- 3.6.4 Sorption -- 3.6.5 Precipitation -- 3.6.6 Adhesion -- 3.6.7 Repellent -- 3.6.8 Cohesion -- 3.6.9 Connectivity -- 3.6.10 Activity -- 3.6.11 Surface-activity -- 3.6.12 Information -- 3.6.13 Competition -- 3.6.14 Nutrition -- 3.6.15 Motility -- 3.6.16 Transportation.
1.3.5 Which are the interactions of the various EPS components? -- 1.3.6 What is the function of complex EPS components? -- 1.3.7 Which EPS components contribute to matrix stability and how can we predict it? -- 1.4 Chaos and Function - Self-Organization -- Acknowledgements -- References -- Chapter 2: EPS - a complex mixture -- Abstract -- 2.1 Introduction -- 2.2 Microbial Polysaccharide Composition -- 2.3 Polysaccharide Structure -- 2.4 Physical Properties -- 2.5 Polysaccharide Interactions -- References -- Chapter 3: The extracellular matrix - an intractable part of biofilm systems -- Abstract
3.1 Introduction -- 3.2 Challenges -- 3.3 Biofilm Matrix Analysis -- 3.4 Biofilm Matrix Constituents -- 3.4.1 Polysaccharides -- 3.4.2 Proteins -- 3.4.3 Amyloids -- 3.4.4 Extracellular nucleic acids -- 3.4.5 Amphiphilic compounds -- 3.4.6 Membrane vesicles -- 3.4.7 Refractory compounds -- 3.5 Bacterial Extracellular Biology -- 3.6 Biofilm Matrix Functionality -- 3.6.1 Architecture -- 3.6.2 Protection -- 3.6.3 Cryo- and Osmo-protection -- 3.6.4 Sorption -- 3.6.5 Precipitation -- 3.6.6 Adhesion -- 3.6.7 Repellent -- 3.6.8 Cohesion -- 3.6.9 Connectivity -- 3.6.10 Activity -- 3.6.11 Surface-activity
3.6.12 Information -- 3.6.13 Competition -- 3.6.14 Nutrition -- 3.6.15 Motility -- 3.6.16 Transportation -- 3.6.17 Communication -- 3.6.18 Conduction -- 3.6.19 Redox-activity -- 3.6.20 Dispersion -- 3.7 Emerging Views of the Biofilm Matrix -- References -- Chapter 4: The transition from bacterial adhesion to the production of EPS and biofilm formation -- Abstract -- 4.1 Introduction -- 4.2 The Transition from Bacterial Adhesion to Biofilm Formation -- 4.3 Bacterial Surface Sensing and Cell Wall Deformation -- 4.4 Methods to Study Bacterial Cell Wall Deformation
4.4.1 Macroscopic bio-optical fluorescence imaging -- 4.4.2 Atomic force microscopy -- 4.4.3 Surface thermodynamic approach -- 4.5 Bacterial Surface Sensing and the Role of EPS in Biofilms -- 4.5.1 Bacterial surface sensing and EPS production -- 4.5.2 Role of EPS in biofilms -- 4.5.2.1 EPS and resistance of biofilms against mechanical attack -- 4.5.2.2 EPS and resistance of biofilms against chemical attack -- 4.5.2.3 Lubricating properties of EPS in biofilms -- 4.6 Methods to Study Biofilm Composition and Structure -- 4.6.1 Microscopic structure of biofilms -- 4.6.2 Composition of biofilms
4.6.3 Viscoelastic properties of biofilm
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