514465808

The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods / Anne Ria Winkelmann, Katrin Bankov, Jens Müller-von der Grün, Jindrich Cinatl, Peter Johannes Wild, Stefan Vallo, Melanie Christin Demes

Winkelmann, Ria,i1984- [Verfasser] ; Bankov, Katrin,i1986- [Verfasser] ; Müller-von der Grün, Jens,i1988- [Verfasser] ; Cinatl, Jindrich [Verfasser] ; Wild, Peter Johannes,i1973- [Verfasser] ; Vallo, Stefan,i1980- [Verfasser] ; Demes, Melanie Christin,i1985- [Verfasser]

London : BioMed Central, 29.01.2022

Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg

1 Online-Ressource (12 Seiten)

Enthalten in: BMC urology. Jahrgang 22, Artikel 10. - London : BioMed Central, 29.01.2022. - Seite 1-12. - 10.1186/s12894-022-00962-4. - ISSN 1471-2490

 

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Background: Routine human papillomavirus (HPV) testing is performed in cervival cancer and is required for classification of some head and neck cancers. In penile cancer a statement on HPV association of the carcinoma is required. In most cases p16 immunohistochemistry as a surrogate marker is applied in this setting. Since differing clinical outcomes for HPV positive and HPV negative tumors are described we await HPV testing to be requested more frequently by clinicians, also in the context of HPV vaccination, where other HPV subtypes are expected to emerge. Method: Therefore, a cohort of archived, formalin-fixed paraffin embedded (FFPE) penile neoplasias was stained for p16 and thereafter tested for HPV infection status via PCR based methods. Additionally to Sanger sequencing, we chose LCD-Array technique (HPV 3.5 LCD-Array Kit, Chipron; LCD-Array) for the detection of HPV in our probes expecting a less time consuming and sensitive HPV test for our probes. Results: We found that LCD-Array is a sensitive and feasible method for HPV testing in routine diagnostics applicable to FFPE material in our cohort. Our cohort of penile carcinomas and carcinomas in situ was associated with HPV infection in 61% of cases. We detected no significant association between HPV infection status and histomorphological tumor characteristics as well as overall survival. Conclusions: We showed usability of molecular HPV testing on a cohort of archived penile carcinomas. To the best of our knowledge, this is the first study investigating LCD-Array technique on a cohort of penile neoplasias.