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Elastin imaging enables noninvasive staging and treatment monitoring of kidney fibrosis / Qinxue Sun, Maike Baues, Barbara M. Klinkhammer, Josef Ehling, Sonja Djudjaj, Natascha I. Drude, Christoph Daniel, Kerstin Amann, Rafael Kramann, Hyojin Kim, Julio Saez-Rodriguez, Ralf Weiskirchen, David C. Onthank, Rene M. Botnar, Fabian Kiessling, Jürgen Floege, Twan Lammers, Peter Boor

Sun, Qinxue, 1981- [Verfasserin/Verfasser]

Baues, Maike [Verfasserin/Verfasser] ; Klinkhammer, Barbara M. [Verfasserin/Verfasser] ; Ehling, Josef [Verfasserin/Verfasser] ; Djudjaj, Sonja [Verfasserin/Verfasser] ; Drude, Natascha I. [Verfasserin/Verfasser] ; Daniel, Christoph [Verfasserin/Verfasser] ; Amann, Kerstin [Verfasserin/Verfasser] ; Kramann, Rafael [Verfasserin/Verfasser] ; Kim, Hyojin, 1987- [Verfasserin/Verfasser] ; Sáez Rodríguez, Julio, 1978- [Verfasserin/Verfasser] ; Weiskirchen, Ralf [Verfasserin/Verfasser] ; Onthank, David C. [Verfasserin/Verfasser] ; Botnar, Rene M. [Verfasserin/Verfasser] ; Kiessling, Fabian [Verfasserin/Verfasser] ; Floege, Jürgen [Verfasserin/Verfasser] ; Lammers, Twan [Verfasserin/Verfasser] ; Boor, Peter [Verfasserin/Verfasser]

Science translational medicine : integrating medicine and science . - Washington, DC : AAAS, 2009-. - 11(2019,486) Artikel-Nummer eaat4865, 14 Seiten

Englisch

Gesehen am 26.08.2019

Digital Object Identifier (DOI): 10.1126/scitranslmed.aat4865

Finding fibrosis - Excessive extracellular matrix deposition in the kidney impairs renal function, contributing to chronic kidney disease. As an alternative to needle-based biopsies used to diagnose and stage kidney disease, Sun et al. used an elastin-specific imaging agent, ESMA, to noninvasively analyze kidney fibrosis. Elastin was overexpressed in renal tissue from patients with kidney fibrosis, and ESMA identified fibrotic areas in human kidneys ex vivo. Similar results were seen in vivo using three mouse models of kidney fibrosis. ESMA could be used to track the progression of renal fibrosis and response to imatinib therapy in mice. These results support the use of ESMA-based imaging for noninvasive renal fibrosis monitoring. - Fibrosis is the common endpoint and currently the best predictor of progression of chronic kidney diseases (CKDs). Despite several drawbacks, biopsies remain the only available means to specifically assess the extent of renal fibrosis. Here, we show that molecular imaging of the extracellular matrix protein elastin allows for noninvasive staging and longitudinal monitoring of renal fibrosis. Elastin was hardly expressed in healthy mouse, rat, and human kidneys, whereas it was highly up-regulated in cortical, medullar, and perivascular regions in progressive CKD. Compared to a clinically relevant control contrast agent, the elastin-specific magnetic resonance imaging agent ESMA specifically detected elastin expression in multiple mouse models of renal fibrosis and also in fibrotic human kidneys. Elastin imaging allowed for repetitive and reproducible assessment of renal fibrosis, and it enabled longitudinal monitoring of therapeutic interventions, accurately capturing anti-fibrotic therapy effects. Last, in a model of reversible renal injury, elastin imaging detected ensuing fibrosis not identifiable via routine assessment of kidney function. Elastin imaging thus has the potential to become a noninvasive, specific imaging method to assess renal fibrosis. - Elastin-specific MRI allows quantitative and longitudinal renal fibrosis assessment and monitoring of treatment efficacy. - Elastin-specific MRI allows quantitative and longitudinal renal fibrosis assessment and monitoring of treatment efficacy.