1800969635

Influenza A virus matrix protein M1 : structural determinants of membrane binding and protein- induced deformation / vorgelegt von Ismail Dahmani ; Betreuer: Salvatore Chiantia, Michael Veit, Oleg V. Batishchev

Chiantia, Salvatore [AkademischeR BetreuerIn] ; Veit, Michael [AkademischeR BetreuerIn] ; Batishchev, Oleg V. [AkademischeR BetreuerIn]

Englisch

Potsdam, 2020

1 Online-Ressource (X, 149 Seiten, 5252 KB) : Illustrationen, Diagramme

Übersetzung des Haupttitels: Influenza-A-Virus-Matrixprotein M1

Erscheint auch als: Druck-Ausgabe- Influenza A virus matrix protein M1. - Potsdam, 2020. - X, 149 Seiten

Dissertation, Universität Potsdam, 2021

URN: urn:nbn:de:kobv:517-opus4-527409

DOI: 10.25932/publishup-52740

Dewey Dezimal-Klassifikation: 616.9;

Influenza A virus (IAV) is a pathogen responsible for severe seasonal epidemics threatening human and animal populations every year. During the viral assembly process in the infected cells, the plasma membrane (PM) has to bend in localized regions into a vesicle towards the extracellular side. Studies in cellular models have proposed that different viral proteins might be responsible for inducing membrane curvature in this context (including M1), but a clear consensus has not been reached. M1 is the most abundant protein in IAV particles. It plays an important role in virus assembly and budding at the PM. M1 is recruited to the host cell membrane where it associates with lipids and other viral proteins. However, the details of M1 interactions with the cellular PM, as well as M1-mediated membrane bending at the budozone, have not been clarified. In this work, we used several experimental approaches to analyze M1-lipids and M1-M1 interactions. By performing SPR analysis, we quantified membrane association for full-length M1 and ...