1816731226

Uncovering the interplay between nutrient availability and cellulose biosynthesis inhibitor activity / Michael Ogden

Willmitzer, Lothar *1952-* [AkademischeR BetreuerIn] ; Roessner, Ute *1971-* [AkademischeR BetreuerIn] ; Hoefgen, Rainer [AkademischeR BetreuerIn]

Englisch

Potsdam ; Melbourne, March 2022

Potsdam ; Melbourne

xi, 127 Seiten : Illustrationen, Diagramme

Dissertation, University of Potsdam, 2022

Dissertation, The University of Melbourne, 2022

*Zellwandbildung / Nährstoffversorgung

All plant cells are surrounded by a dynamic, carbohydrate-rich extracellular matrix known as the cell wall. Nutrient availability affects cell wall composition via uncharacterized regulatory mechanisms, and cellulose deficient mutants develop a hypersensitive root response to growth on high concentrations of nitrate. Since cell walls account for the bulk of plant biomass, it is important to understand how nutrients regulate cell walls. This could provide important knowledge for directing fertilizer treatments and engineering plants with higher nutrient use efficiency. The direct effect of nitrate on cell wall synthesis was investigated through growth assays on varying concentrations of nitrate, measuring cellulose content of roots and shoots, and assessing cellulose synthase activity (CESA) using live cell imaging with spinning disk confocal microscopy. A forward genetic screen was developed to isolate mutants impaired in nutrient-mediated cell wall regulation, revealing that cellulose biosynthesis inhibitor (CBI) activity is modulated by nutrient availability. [...]

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