1878600656     

Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T : evaluation of a modeling workflow / Florian Selt, Ahmed El Damaty, Martin U. Schuhmann, Romain Sigaud, Jonas Ecker, Philipp Sievers, Daniela Kocher, Christel Herold-Mende, Ina Oehme, Andreas von Deimling, Stefan M. Pfister, Felix Sahm, David T.W. Jones, Olaf Witt, Till Milde

Selt, Florian, 1984- [Verfasserin/Verfasser]

El Damaty, Ahmed [Verfasserin/Verfasser] ; Schuhmann, Martin U. [Verfasserin/Verfasser] ; Sigaud, Romain [Verfasserin/Verfasser] ; Ecker, Jonas, 1988- [Verfasserin/Verfasser] ; Sievers, Philipp [Verfasserin/Verfasser] ; Kocher, Daniela [Verfasserin/Verfasser] ; Herold-Mende, Christel [Verfasserin/Verfasser] ; Oehme, Ina, 1974- [Verfasserin/Verfasser] ; Deimling, Andreas von, 1959- [Verfasserin/Verfasser] ; Pfister, Stefan, 1974- [Verfasserin/Verfasser] ; Sahm, Felix, 1984- [Verfasserin/Verfasser] ; Jones, David T. W. [Verfasserin/Verfasser] ; Witt, Olaf, 1965- [Verfasserin/Verfasser] ; Milde, Till [Verfasserin/Verfasser]

Journal of neuro-oncology. - Dordrecht [u.a.] : Springer Science + Business Media B.V ; Dordrecht [u.a.] : Kluwer, 1983-. - Bd. 165 (2023), 3 (November), S. 467-478 = 12 S. : Illustrationen

Englisch

Gesehen am 22.01.2024

Elektronische Ressource: Zugang beim Produzenten (Lizenzangabe: Lizenzpflichtig)

Digital Object Identifier (DOI): 10.1007/s11060-023-04500-6

Circumvention of OIS ; In-vitro models ; Inducible SV40 large T ; Pediatric low-grade glioma cell lines ; Pilocytic astrocytoma

Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-grade tumors fail in PA due to oncogene-induced senescence (OIS) driving tumor cells into arrest. Here, we describe a PA modeling workflow using the Simian Virus large T antigen (SV40-TAg) to circumvent OIS.