219616558

Chemoresistance acquisition induces a global shift of expression of aniogenesis-associated genes and increased pro-angogenic activity in neuroblastoma cells [Elektronische Ressource] / Martin Michaelis, Denise Klassert, Susanne Barth, Tatyana Suhan, Rainer Breitling, Bernd Mayer, Nora Hinsch, Hans W Doerr, Jaroslav Cinatl and Jindrich Cinatl jr

Michaelis, Martin,i1972- ; Klassert, Denise ; Barth, Susanne,i1974- ; Suhan, Tatyana ; Breitling, Rainer,i1971- ; Mayer, Bernd ; Hinsch, Nora,i1976- ; Doerr, Hans W. ; Cinatl, Jaroslav ; Cinatl, Jindrich jr.

Background: Chemoresistance acquisition may influence cancer cell biology. Here, bioinformatics analysis of gene expression data was used to identify chemoresistance-associated changes in neuroblastoma biology. Results: Bioinformatics analysis of gene expression data revealed that expression of angiogenesis-associated genes significantly differs between chemosensitive and chemoresistant neuroblastoma cells. A subsequent systematic analysis of a panel of 14 chemosensitive and chemoresistant neuroblastoma cell lines in vitro and in animal experiments indicated a consistent shift to a more pro-angiogenic phenotype in chemoresistant neuroblastoma cells. The molecular mechanims underlying increased pro-angiogenic activity of neuroblastoma cells are individual and differ between the investigated chemoresistant cell lines. Treatment of animals carrying doxorubicin-resistant neuroblastoma xenografts with doxorubicin, a cytotoxic drug known to exert anti-angiogenic activity, resulted in decreased tumour vessel formation and growth indicating chemoresistance-associated enhanced pro-angiogenic activity to be relevant for tumour progression and to represent a potential therapeutic target. Conclusions: A bioinformatics approach allowed to identify a relevant chemoresistance-associated shift in neuroblastoma cell biology. The chemoresistance-associated enhanced pro-angiogenic activity observed in neuroblastoma cells is relevant for tumour progression and represents a potential therapeutic target.

Molecular cancer. - London : Biomed Central, 2002-. - Online-Ressource. - ISSN 1476-4598. - Band 8, Heft 80 (2009)

 

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