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Inflammatory and angiogenic protein detection in the human vitreous [Elektronische Ressource] : cytometric bead assay / M. J. Koss, M. Pfister, and F. H. Koch

Koss, Michael Janusz,i1979- ; Pfister, Marcel,i1979- ; Koch, Frank H.

Introduction. To evaluate clinical feasibility and reproducibility of cytometric bead assay (CBA) in nondiluted vitreous samples of patients with age-related macular degeneration (ARMD), diabetic macular edema (DME), and central retinal vein occlusion (CRVO). Methods. Twelve patients from a single clinics day qualified for intravitreal injections (ARMD n = 6, DME n = 3, CRVO n = 3) and underwent a combination treatment including a single-site 23 gauge core vitrectomy which yielded a volume of 0.6 mL undiluted vitreous per patient. Interleukin-6 (IL-6), vascular endothelial growth factor isoform A (VEGF-A), and monocyte chemo-attractant protein-1 (MCP-1) were assessed directly from 0.3 mL at the same day (fresh samples). ...

Journal of ophthalmology. - New York, NY : Hindawi, 2009-. - Online-Ressource. - ISSN 2090-0058. - Heft 459251 (2011)

 

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