Optogenetics

Published by De Gruyter 2013

Optogenetics

Edited by: Peter Hegemann and Stephan Sigrist

In the series Dahlem Workshop Reports

https://doi.org/10.1515/9783110270723

Overview
Contents

About this book

Optogenetics combines genetic engineering with optics to observe and control the function of cells using light, with clinical implications for restoration of vision and treatment of neurological diseases. As a new discipline much of the basic science and methods are currently under investigation and active development, thus there is a strong need for introductory literature in this field. This graduate level textbook provides an overview of the field of optogenetics in 5 concise chapters: Optogenetic tools, Applications in cellular systems, Mapping neuronal networks, Clinical applications and Restoration of vision and hearing. The concept and content was developed with top international researchers and students at a prestigious Dahlem Conference workshop.

A concise overview of the methodology, basic research and new clinical applications for optogenetics
Content developed with top international scientists and students in a "Dahlem Conference" workshop
Color illustrations for better visualization of complex structures

Author / Editor information

Peter Hegemann, Humboldt University, Berlin, Germany; Stephan Sigrist, Free University, Berlin, Germany.

Supplementary Materials

Contents

Topics

Frontmatter Publicly Available Download PDF	I
Content Publicly Available Download PDF	IX
Introduction Requires Authentication Unlicensed Licensed Download PDF	1
1 The biophysics and engineering of signaling photoreceptors Keith Moffat, Feng Zhang, Klaus Hahn and Andreas Möglich Requires Authentication Unlicensed Licensed Download PDF	7
2 Current challenges in optogenetics Kelly A. Zalocusky, Lief E. Fenno and Karl Deisseroth Requires Authentication Unlicensed Licensed Download PDF	23
3 Challenges and opportunities for optochemical genetics Ehud Y. Isacoff, Richard H. Kramer and Dirk Trauner Requires Authentication Unlicensed Licensed Download PDF	35
4 Optogenetic imaging of neural circuit dynamics using voltage-sensitive fluorescent proteins: potential, challenges and perspectives Thomas Knöpfel Requires Authentication Unlicensed Licensed Download PDF	47
5 Why optogenetic “control” is not (yet) control Gero Miesenböck Requires Authentication Unlicensed Licensed Download PDF	55
6 Optogenetic actuation, inhibition, modulation and readout for neuronal networks generating behavior in the nematode Caenorhabditis elegans Mario de Bono, William R. Schafer and Alexander Gottschalk Requires Authentication Unlicensed Licensed Download PDF	61
7 Putting genetics into optogenetics: knocking out proteins with light Matt L. Labella, Stephan Sigrist and Erik M. Jørgensen Requires Authentication Unlicensed Licensed Download PDF	79
8 Optogenetic approaches in behavioral neuroscience André Fiala Requires Authentication Unlicensed Licensed Download PDF	91
9 Combining genetic targeting and optical stimulation for circuit dissection in the zebrafish nervous system Fumi Kubo and Herwig Baier Requires Authentication Unlicensed Licensed Download PDF	101
10 Optogenetic analysis of mammalian neural circuits Thomas G. Oertner, Fritjof Helmchen, Luis de Lecea, Heinz Beck, Arthur Konnerth, Benjamin Kaupp, Thomas Knöpfel, Hiromu Yawo and Michael Häusser Requires Authentication Unlicensed Licensed Download PDF	109
11 Optogenetics to benefit human health: opportunities and challenges Viviana Gradinaru Requires Authentication Unlicensed Licensed Download PDF	127
12 Optogenetic tools for controlling neural activity: molecules and hardware Edward S. Boyden Requires Authentication Unlicensed Licensed Download PDF	133
13 In vivo application of optogenetics in rodents Ada Eban-Rothschild, Clara Touriño and Luis de Lecea Requires Authentication Unlicensed Licensed Download PDF	143
14 Potential of optogenetics in deep brain stimulation V. Sturm Requires Authentication Unlicensed Licensed Download PDF	157
15 Optogenetic approaches for vision restoration Zhuo-Hua Pan, Botond Roska and José-Alain Sahel Requires Authentication Unlicensed Licensed Download PDF	161
16 Restoration of vision – the various approaches Eberhart Zrenner and Birgit Lorenz Requires Authentication Unlicensed Licensed Download PDF	173
17 Optogenetic approaches to cochlear prosthetics for hearing restoration Tobias Moser Requires Authentication Unlicensed Licensed Download PDF	187
18 History in the making: the ethics of optogenetics Sabine Schleiermacher Requires Authentication Unlicensed Licensed Download PDF	193
19 Optogenetics as a new therapeutic tool in medicine? A view from the principles of biomedical ethics Henrik Walter and Sabine Müller Requires Authentication Unlicensed Licensed Download PDF	201
Appendix Requires Authentication Unlicensed Licensed Download PDF	213
Index Requires Authentication Unlicensed Licensed Download PDF	223

(Deutsch)

Language: English
Publisher: De Gruyter
Audience: Graduate students in molecular biology, biophysics, biochemistry, biomedical engineering, molecular medicine, clinical medicine
Pages
- Front matter: 14
- Main content: 226
Illustrations
- Coloured Illustrations: 75
Keywords: Biophysics; Genetic Engineering; Neuroscience; Optics; Vision Restoration

eBook

Published: August 28, 2013
ISBN: 9783110270723

Hardcover

Published: August 29, 2013
ISBN: 9783110270716