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Colorimetric analysis based on solid-phase extraction with sedimentable dispersed particulates: demonstration of concept and application for on-site environmental water analysis

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Abstract

A novel simple and functional colorimetric methodology for on-site environmental water analysis was proposed. This method combines coloration of the analyte and extraction of the colored species on dispersed particulates during their sedimentation in the same container. The whole analysis can be performed within 15 min by comprising the addition of 1 mL of sample solution into a 1.5-mL microtube containing the powders of coloring reagents and the sedimentable fine particulates as an adsorbent. The analyte is determined by comparing the sediment color with the standard color by visual inspection or the color information of the photo image. The potential of this methodology was demonstrated through developing colorimetry for Fe2+ with o-phenanthroline, NO2− by azo-dye formation, HCHO by the MBTH method, and PO43− by the 4-aminoantipyrine method based on the enzyme reactions. The material, size, amount of the adsorbent particles, and other conditions were optimized for each analytes. The advantages of the methodology were as follows: high sensitivity, easy controllability of the sensitivity over the wide range by the amount, size, and material of the particulates, lower interference from the colored matrix components due to obtaining the color data from not the aqueous phase but the sedimented particulates, and acceleration of the color development rate by the particulates as seen in NO2− determination as consequence shorten the operation time. A simple device equipped with twin cells was proposed for on-site analysis which contains two successive different coloring operations. The developed methods were successfully applied to the environmental water samples with the good agreement of the results with those by the usual instrumental methods.

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References

  1. Turnbull LH. A laboratory trial of some modern screening tests for the detection of glucose and protein in urine. Br J Ind Med. 1959. https://doi.org/10.1136/oem.16.4.326.

  2. Kaneko E. Development of visual analytical methods for trace determination. Anal Sci. 2004. https://doi.org/10.2116/analsci.20.247.

  3. Yang Y, Noviana E, Nguyen MP, Geiss BJ, Dandy DS, Henry CS. Paper-based microfluidic devices: emerging themes and applications. Anal Chem. 2017. https://doi.org/10.1021/acs.analchem.6b04581.

  4. Cate DM, Adkins JA, Mettakoonpitak J, Henry CS. Recent developments in paper-based microfluidic devices. Anal Chem. 2015. https://doi.org/10.1021/ac503968p.

  5. Shimada Y, Kaneta T. Highly sensitive paper-based analytical devices with the introduction of a large-volume sample via continuous flow. Anal Sci. 2018. https://doi.org/10.2116/analsci.34.65.

  6. Jayawardane BM, Wei S, McKelvie ID, Kolev SD. Microfluidic paper-based analytical device for the determination of nitrite and nitrate. Anal Chem. 2014. https://doi.org/10.1021/ac5013249.

  7. Quinn CW, Cate DM, Miller-Lionberg DD, Reilly T, Volckens J, Henry CS. Solid-phase extraction coupled to a paper-based technique for trace copper detection in drinking water. Environ Sci Technol. 2018. https://doi.org/10.1021/acs.est.7b05436.

  8. Jayawardane BM, McKelvie ID, Kolev SD. Development of a gas-diffusion microfluidic paper-based analytical device (μPAD) for the determination of ammonia in wastewater samples. Anal Chem. 2015. https://doi.org/10.1021/acs.analchem.5b00125.

  9. Cioffi A, Mancini M, Gioia V, Cinti S. Office paper-based electrochemical strips for organophosphorus pesticide monitoring in agricultural soil. Environ Sci Technol. 2021. https://doi.org/10.1021/acs.est.1c01931.

  10. Li M, Cao R, Nilghaz A, Guan L, Zhang X, Shen W. “Periodic-table-style” paper device for monitoring heavy metals in water. Anal Chem. 2015. https://doi.org/10.1021/acs.analchem.5b00040.

  11. Yamaguchi A, Miyaguchi H, Ishida A, Tokeshi M. Paper-based analytical device for the on-site detection of nerve agents. ACS Appl Bio Mater. 2021. https://doi.org/10.1021/acsabm.1c00655.

  12. Resano M, Belarra MA, García-Ruiz E, Aramendía M, Rello L. Dried matrix spots and clinical elemental analysis. Current status, difficulties, and opportunities. TrAC-Trends Anal Chem. 2018. https://doi.org/10.1016/j.trac.2017.12.004.

  13. Yang J, Wang K, Xu H, Yan W, Jin Q, Cui D. Detection platforms for point-of-care testing based on colorimetric, luminescent and magnetic assays: a review. Talanta. 2019. https://doi.org/10.1016/j.talanta.2019.04.054.

  14. Morbioli GG, Mazzu-Nascimento T, Stockton AM, Carrilho E. Technical aspects and challenges of colorimetric detection with microfluidic paper-based analytical devices (μPADs) - A review. Anal Chim Acta. 2017. https://doi.org/10.1016/j.aca.2017.03.037.

  15. Liu D, Wang J, Wu L, Huang Y, Zhang Y, Zhu M, Wang Y, Zhu Z, Yang C. Trends in miniaturized biosensors for point-of-care testing. TrAC-Trends Anal Chem. 2020. https://doi.org/10.1016/j.trac.2019.115701.

  16. Kawakubo S, Naito A, Fujihara A, Iwatsuki M. Field determination of trace iron in fresh water samples by visual and spectrophotometric methods. Anal Sci. 2004. https://doi.org/10.2116/analsci.20.1159.

  17. Watanabe Y, Kurata Y, Ono Y, Hosomi M. On-site screening of As, Cr and Cu in waste woods using commercial analytical kits. J Environ Chem. 2004. https://doi.org/10.5985/jec.14.495.

  18. Meyerhoff ME, Fu B, Bakker E, Yun J-H, Yang VC. Polyion-sensitive membrane electrodes for biomedical analysis. Anal Chem Soc. 1996. https://doi.org/10.1021/ac9618536.

  19. Thurman EM, Mills MS. Solid-phase extraction: principles and practice. New York: Wiley; 1998.

    Google Scholar 

  20. Erger C, Schmidt TC. Disk-based solid-phase extraction analysis of organic substances in water. TrAC-Trends Anal Chem. 2014. https://doi.org/10.1016/j.trac.2014.05.006.

  21. Taguchi S, Ito-oka E, Goto K. Application of the organic solvent-soluble membrane filter to the preconcentration and spectrophotometric determination of traces of phosphorus in water. Bunseki Kagaku. 1984. https://doi.org/10.2116/bunsekikagaku.33.8_453.

  22. Shida J, Masuda I. Photoacoustic spectrometric determination of trace iron(II) after preconcentration on a membrane filter with a finely pulverized anion-exchange resin. Anal Sci. 1998. https://doi.org/10.2116/analsci.14.333.

  23. Gu X, Zhou T, Qi D. Determination of trace nitrite ion in water by spectrophotometric method after preconcentration on an organic solvent-soluble membrane filter. Talanta. 1996. https://doi.org/10.1016/0039-9140(95)01686-4.

  24. Takahashi Y, Danwittayakul S, Suzuki TM. Dithizone nanofiber-coated membrane for filtration-enrichment and colorimetric detection of trace Hg(II) ion. Analyst. 2009. https://doi.org/10.1039/B816461D.

  25. Mizuguchi H, Matsuda Y, Mori T, Uehara A, Ishikawa Y, Endo M, Shida J. Visual colorimetry for trace antimony(V) by ion-pair solid-phase extraction with bis[2-(5-chloro-2-pyridylazo)-5-diethylaminophenolato]cobalt(III) on a PTFE type membrane filter. Anal Sci. 2008. https://doi.org/10.2116/analsci.24.219.

  26. Mizuguchi H, Zhang VF, Onodera H, Nishizawa S, Shida J. On-site determination of trace nickel in liquid samples for semiconductor manufacturing by highly sensitive solid-phase colorimetry with α-furil dioxime. Chem Lett. 2008. https://doi.org/10.1246/cl.2008.792.

  27. Tang X, Tan Y, Zhu H, Zhao K, Shen W. Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli. Appl Environ Microbiol. 2009. https://doi.org/10.1128/AEM.02376-08.

  28. Murai K, Okano M, Kuramitz H, Hata N, Kawakami T, Taguchi S. Investigation of formaldehyde pollution of tap water and rain water using a novel visual colorimetry. Water Sci Technol. 2008. https://doi.org/10.2166/wst.2008.470.

  29. Kasahara I, Terai R, Murai Y, Hata N, Taguchi S, Goto K. Spectrophotometric determination of traces of silicon in water after collection as silicomolybdenum blue on an organic-solvent-soluble membrane filter. Anal Chem. 1987. https://doi.org/10.1021/ac00132a023.

  30. Taguchi S, Kakinuma A, Kasahara I. Electrothermal atomic absorption spectrophotometric determination of copper-reactive pesticides in water after preconcentration with a solvent-soluble membrane filter. Anal Sci. 1999. https://doi.org/10.2116/analsci.15.1149.

  31. Okazaki T, Kuramitz H, Hata N, Shigeru T, Murai K, Okauchi K. Visual colorimetry for determination of trace arsenic in groundwater based on improved molybdenum blue spectrophotometry. Anal Methods. 2015. https://doi.org/10.1039/C4AY03021D.

  32. Okazaki T, Wang W, Kuramitz H, Hata N, Taguchi S. Molybdenum blue spectrophotometry for trace arsenic in ground water using a soluble membrane filter and calcium carbonate column. Anal Sci. 2013. https://doi.org/10.2116/analsci.29.67.

  33. Murai K, Honda H, Okumura H, Okauchi K. On-site visual colorimetry for dissolved trace manganese after extraction into ion-associate phase retained on membrane filter. Bunseki Kagaku. 2011. https://doi.org/10.2116/bunsekikagaku.44.505.

  34. Murai K, Honda H, Okumura H, Okauchi S. On-site colorimetric determination of trace arsenic in water using a syringe filter as a separation and pre-concentration device. Bunseki Kagaku. 2019. https://doi.org/10.2116/bunsekikagaku.68.465.

  35. Serra AM, Estela JM, Coulomb B, Boudenne JL, Cerdà V. Solid phase extraction – multisyringe flow injection system for the spectrophotometric determination of selenium with 2,3-diaminonaphthalene. Talanta. 2010. https://doi.org/10.1016/j.talanta.2009.12.045.

  36. Taguchi S, Ito-Oka E, Masuyama K, Kasahara I, Goto K. Application of organic solvent-soluble membrane filters in the preconcentration and determination of trace elements: spectrophotometric determination of phosphorus as phosphomolybdenum blue. Talanta. 1985. https://doi.org/10.2116/bunsekikagaku.33.8_453.

  37. Gilcreas FW. Standard methods for the examination of water and waste water. Am J Public Health Nations Health. 1966;56:387–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. WHO. Guidelines for drinking-water quality: fourth edition incorporating the first and second addenda. 2022. https://www.who.int/publications/i/item/9789240045064. Accessed 28 Sep 2022.

  39. Sawicki E, Hauser TR, Stanley TW, Elbert W. The 3-methyl-2-benzothiazolone hydrazone test: sensitive new methods for the detection, rapid estimation, and determination of aliphatic aldehydes. Anal Chem. 1961. https://doi.org/10.1021/ac60169a028.

  40. Morita E, Nakamura E. Solid-phase extraction of antipyrine dye for spectrophotometric determination of phenolic compounds in water. Anal Sci. 2011. https://doi.org/10.2116/analsci.27.489.

  41. Isobe K, Yamada H, Soejima Y, Otsuji S. A rapid enzymatic assay for total blood polyamines. Clin Biochem. 1987. https://doi.org/10.1016/S0009-9120(87)80113-3.

  42. Mohammadnejad P, Asl SS, Aminzadeh S, Haghbeen K. A new sensitive spectrophotometric method for determination of saliva and blood glucose. Spectrochim Acta Part A Mol Biomol Spectrosc. 2020. https://doi.org/10.1016/j.saa.2019.117897.

  43. Spayd RW, Bruschi B, Burdick BA, Dappen GM, Elkenberry JN, Esders TW, Figueras J, Goodhue CT, LaRossa DD, Nelson RW, Rand RN, Tai-Wing W. Multilayer film elements for clinical analysis: applications to representative chemical determinations. Semant Sch. 1978. https://doi.org/10.1093/clinchem/24.8.1343.

  44. Racicot JM, Mako TL, Olivelli A, Levine M. A paper-based device for ultrasensitive, colorimetric phosphate detection in seawater. Sensors. 2020;2020. https://doi.org/10.3390/S20102766.

  45. PackTest Phosphate (Low Range)- Kyoritsu Chemical-Check Lab. https://kyoritsu-lab.co.jp/products/wak_po4_d. Accessed 28 September 2022.

  46. Guilfoyle A, Maher MJ, Rapp M, Clarke R, Harrop S, Jormakka M. Structural basis of GDP release and gating in G protein coupled Fe2+ transport. EMBO J. 2009. https://doi.org/10.1038/EMBOJ.2009.208.

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Funding

This work was supported by JST SPRING, Grant Number JPMJSP2145and the Japan Society for the Promotion of Science (JSPS) via a Grant-in-Aid for Scientific Research (Grant JP 22H01624).

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Authors and Affiliations

  1. Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering for Research, University of Toyama, Gofuku, Toyama, 930-8555, Japan

    Nozomi Kohama, Koki Matsuhira, Takuya Okazaki, Kazuto Sazawa, Noriko Hata, Hideki Kuramitz & Shigeru Taguchi

  2. Department of Applied Chemistry, School of Science and Technology, Meiji University, Kawasaki, Kanagawa, 214-8571, Japan

    Takuya Okazaki

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  1. Nozomi Kohama
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Kohama, N., Matsuhira, K., Okazaki, T. et al. Colorimetric analysis based on solid-phase extraction with sedimentable dispersed particulates: demonstration of concept and application for on-site environmental water analysis. Anal Bioanal Chem 414, 8389–8400 (2022). https://doi.org/10.1007/s00216-022-04375-y

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