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Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous leaf extract of Hardwickia binata: their characterizations and biological applications

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Abstract

The synthesis of ZnO NPs (zinc oxide nanoparticles) from Hardwickia binata leaves extract using zinc acetate NP (nanoparticle) was verified by a colour change and it was assessed using a UV-Vis spectrophotometer at 365 nm. FTIR (Fourier transform infra-red) analysis was done to demonstrate the existence of several functional groups. XRD (X-ray diffraction) findings support to find the structure of ZnO-NPs. SEM (scanning electron microscopy) data confirmed their synthesis ZnO NPs and its shape. Size range of particles was confirmed by using TEM (transmission electron microscopy). Staphylococcus aureus shows high inhibition compared to other bacterial strains. Antioxidant activity shows highest inhibition in DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) compared to ABTS (2.2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and hydroxyl radical scavenging assay. Anti-inflammatory assay shows highest level of inhibition in HRBC (human red blood cell) membrane stabilization and albumin denaturation. Anti-cancer activity of synthesised ZnO NPs was performed in A431 Human carcinoma cancer cell line and HepG2 liver cancer cell line to evaluate the cytotoxicity of biosynthesized ZnO-NPs. From these findings, it should be noted that synthesised NPs can be utilized for a variety of global biomedical applications in the future.

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Acknowledgements

The author acknowledges Periyar University for provided University Research Fellowship (URF/000876/2021), Salem, Tamil Nadu, India. This work was funded by the Researchers Supporting Project Number (RSPD2023R763) King Saud University, Riyadh, Saudi Arabia. The authors also thank to Department of Botany, School of Life Sciences, Periyar University, Salem, Tamil Nadu-636 011, India for providing infrastructural facility. This work was supported by Ming Chi University of Technology, Taishan, Taiwan.

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Authors and Affiliations

  1. Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 636 011, India

    Peraman Manimegalai, Kuppusamy Selvam & Dharmalingam Kirubakaran

  2. Department of Anatomy, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, 600077, India

    Settu Loganathan

  3. Department of Botany, Government Arts College, Dharmapuri, Tamil Nadu, 636 705, India

    Settu Loganathan

  4. Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, 636 011, India

    Muthugounder Subaramanian Shivakumar

  5. Faculty, International PhD Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City, 243303, Taiwan

    Mani Govindasamy

  6. Department of Research and Innovation, Saveetha School of Engineering, SIMATS, Chennai, 602105, India

    Mani Govindasamy

  7. Department of Chemistry, Korea University, Seoul, Seongbuk-gu, Republic of Korea

    Umamaheswari Rajaji

  8. Department of Materials Engineering, Ming Chi University of Technology, 243303, New Taipei City, Taiwan

    Umamaheswari Rajaji

  9. Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Aboud Ahmed Awadh Bahajjaj

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  1. Peraman Manimegalai
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Contributions

Peraman Manimegalai: investigation, conceptualization, methodology, writing—original draft. Kuppusamy Selvam, Settu Loganathan: conceptualization, data curation, writing—original draft, Dharmalingam Kirubakaran, Muthugounder Subaramanian Shivakumar, Mani Govindasamy, Umamaheswari Rajaji, and Aboud Ahmed Awadh Bahajjaj: formal analysis. All authors read and approved the final manuscript.

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Manimegalai, P., Selvam, K., Loganathan, S. et al. Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous leaf extract of Hardwickia binata: their characterizations and biological applications. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04279-6

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