S. aureus MscL Is a Pentamer In Vivo but of Variable Stoichiometries In Vitro: Implications for Detergent-Solubilized Membrane Proteins
Figure 5
The S. aureus MscL stoichiometry change is reversible.
(A) The oligomeric state of SaMscL in 4 mM LDAO was determined using SEC-MALS, which measured a protein mass of 61.3 kDa, consistent with a tetrameric channel (top chromatogram). (B) The SaMscL-LDAO sample was exchanged into C8E5, and the following day the oligomeric state of SaMscL-C8E5 sample was measured by SEC-MALS. Analysis of the SEC-MALS results for the SaMscL-C8E5 sample showed a protein mass of 74.4 kDa, consistent with the channel in a pentameric state.