A Proteomic and Cellular Analysis of Uropods in the Pathogen Entamoeba histolytica
Figure 3
Distribution in functional categories of the proteins present in the E. histolytica uropod extruded fraction.
A. Electrophoretic analysis of proteins from the ConA-uropod complex and from crude extract. A sample of UEF or amoebic extracts (10 µg, U = uropod; A = amoebae) were resolved by SDS-PAGE. The Gal/GalNAc lectin heavy chain (170 kDa) and the Con A (24 kDa) were revealed by western blot. B. Protein identification with LC-MS/MS was followed by proteome comparisons using the BLAST computer program, GO annotations and manual annotations. Two LC-MS/MS experiments were performed. Only proteins identified by at least two peptides in each experiment were taken into account. In all, 104 proteins were present in both experiments and could be analyzed. The entire data set was submitted to Tranche (https://proteomecommons.org/tranche/) database.