A Proteomic and Cellular Analysis of Uropods in the Pathogen Entamoeba histolytica
Figure 4
Cysteine proteinases are present as pro-enzymes and active enzymes in the uropod extruded fractions.
A. Substrate gel electrophoresis of uropod extruded fractions (1 µg of proteins), which were separated by electrophoresis in SDS-PAGE co-polymerized with gelatine. To visualize the cysteine proteinase activity, gels were stained with Coomassie blue. The figure shows the inverted image. B. Cellular localisation of CP-A5, -A1 and -A2 in E. histolytica. Trophozoites were incubated with Con A (green). Upon incubation, the cells were fixed and stained for CP-A5 (up panel) or CP-A1 and -A2 (low panel) with specific antibodies (red). Scale bar: 10 µm.