A Generic Method for Design of Oligomer-Specific Antibodies
Figure 7
Binding to biologically active oligomers of Aβ1–42.
Isolated Aβ1–42 oligomers exert a cytotoxic effect on SH-SY5Y cells, and the attenuating effect of the oligomer-specific mAB-O antibody was evaluated. Cells exposed to Aβ1–42 oligomers are shown with grey bars while controls are depicted in black. Addition of mAB-O in a 1∶5 molar ratio to Aβ1–42 fully attenuates the effect. Addition of Aβ1–10 monomer at a 100-fold molar excess does not interfere with the attenuating effect.