Pathogenic Chlamydia Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division
Fig 3
Chlamydial EBs do not retain PG labeling.
(a) SIM of EDA—DA labeled C. trachomatis inclusions at 22 hpi. Arrowheads indicate locations of EBs. Tissue culture cells were infected with C. trachomatis and placed on a rocker for 2 hours, ensuring asynchronous infection of the cell monolayer. Bacteria were then incubated with 4 mM EDA-DA for 22 hours. (b) Confocal maximum intensity projections of a mature, Chlamydia inclusion (40 hpi) in which bacteria were grown in the presence of 4 mM EDA—DA for the entire developmental cycle. The arrowheads point to EBs (distinguished by their MOMP labeling and smaller size). (c) Confocal maximum intensity projections of Chlamydia-infected cells 4 hpi. EBs used for infection were harvested from cells that had been incubated with EDA—DA for 18 hours. Exogenous EDA—DA (4 mM) was present throughout the EB harvest as well as the subsequent reinfection. MOMP and PG labeling is the same as in Fig 2. Images are all representative of at least three separate experiments. Scale bar = 1 μm.