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Identification of marker-trait associations in the German winter barley breeding gene pool (Hordeum vulgare L.)

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Abstract

A genome-wide association mapping approach for grain yield and traits of high agronomic relevance was carried out on basis of a set of 61 six-rowed and 48 two-rowed German winter barley (Hordeum vulgare L.) cultivars representing breeding progress in the period 1959–2003. Extensive phenotyping was conducted in field trials carried out at 12 locations in 3 years. Heritability was estimated at between 0.45 for grain yield and 0.94 for grains per spike. By using the Illumina Golden Gate Bead Array technology, 833 single nucleotide polymorphisms with an allele frequency higher than 5% were obtained. Linkage disequilibrium on the whole genome extends to 7.35 cM. Based on a mixed linear model approach taking into account the population structure estimated on the basis of 72 simple sequence repeat markers covering the whole barley genome, 91 significant marker-trait associations were detected, corresponding to 48 different genomic regions.

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Acknowledgments

The authors wish to thank the co-operating breeding companies Deutsche Saatveredelung AG, Dieckmann GmbH & Co. KG, Limagrain GmbH, KWS Lochow GmbH, Nordsaat Saatzucht GmbH, Pflanzenzucht Oberlimpurg, Saatzucht Bauer GmbH & Co. KG, Lantmännen SW Seed GmbH, Saatzucht Josef Breun GmbH & Co. KG, Saatzucht Streng GmbH & Co. KG, Secobra Saatzucht GmbH and W. von Borries-Eckendorf GmbH & Co. KG for their support. This work is financed by the German Research Foundation (DFG) as part of the Collaborative Research Centre (SFB) 299 at the University of Giessen and by the Federal Ministry of Education and Research (BMBF) within the framework of GABI-GENOBAR (FKZ 03115066 A–E).

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  1. Jeannette Rode

    Present address: Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Betty-Heimann-Str. 3, 06120, Halle (Saale), Germany

  2. Jutta Ahlemeyer

    Present address: Deutsche Saatveredelung AG (DSV), Weissenburger Str. 5, 59557, Lippstadt, Germany

Authors and Affiliations

  1. Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Jeannette Rode & Frank Ordon

  2. Justus Liebig University, Department of Plant Breeding, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Jutta Ahlemeyer & Wolfgang Friedt

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  1. Jeannette Rode
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Correspondence to Jeannette Rode.

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Rode, J., Ahlemeyer, J., Friedt, W. et al. Identification of marker-trait associations in the German winter barley breeding gene pool (Hordeum vulgare L.). Mol Breeding 30, 831–843 (2012). https://doi.org/10.1007/s11032-011-9667-6

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