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In Vitro Methods for Studying the Mechanisms of Resistance to DNA-Damaging Therapeutic Drugs

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Cancer Drug Resistance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1395))

Abstract

Most commonly used anticancer drugs exert their effects mainly by causing DNA damage. The enhancement in DNA damage response (DDR) is considered a key mechanism that enables cancer cells to survive through eliminating the damaged DNA lesions and thereby developing resistance to DNA-damaging agents. This chapter describes the four experimental approaches for studying DDR and genotoxic drug resistance, including the use of γ-H2AX and comet assays to monitor DNA damage and repair capacity as well as the use of clonogenic and β-galactosidase staining assays to assess long-term cell fate after DNA-damaging treatment. Finally, we also present examples of these methods currently used in our laboratory for studying the role of FOXM1 in DNA damage-induced senescence and epirubicin resistance.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-3347-1_18

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-3347-1_18

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Authors and Affiliations

  1. Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK

    Pasarat Khongkow, Anna W. Middleton, Jocelyn P.-M. Wong, Navrohit K. Kandola, Mesayamas Kongsema, Gabriela Nestal de Moraes, Ana R. Gomes & Eric W.-F. Lam

Authors
  1. Pasarat Khongkow
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  2. Anna W. Middleton
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  3. Jocelyn P.-M. Wong
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  4. Navrohit K. Kandola
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  5. Mesayamas Kongsema
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  6. Gabriela Nestal de Moraes
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  7. Ana R. Gomes
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  8. Eric W.-F. Lam
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Corresponding author

Correspondence to Eric W.-F. Lam .

Editor information

Editors and Affiliations

  1. New University of Lisbon, Lisbon, Portugal

    Jose Rueff

  2. New University of Lisbon, Lisbon, Portugal

    António Sebastião Rodrigues

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Khongkow, P. et al. (2016). In Vitro Methods for Studying the Mechanisms of Resistance to DNA-Damaging Therapeutic Drugs. In: Rueff, J., Rodrigues, A. (eds) Cancer Drug Resistance. Methods in Molecular Biology, vol 1395. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3347-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3347-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3345-7

  • Online ISBN: 978-1-4939-3347-1

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